PMID- 12951423
OWN - NLM
STAT- MEDLINE
DCOM- 20031205
LR  - 20190605
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 65
IP  - 8
DP  - 2003 Aug
TI  - Concentrations and specific antibodies to staphylococcal enterotoxin-C and toxic 
      shock syndrome toxin-1 in bovine mammary gland secretions, and inflammatory 
      response to the intramammary inoculation of these toxins.
PG  - 899-906
AB  - To investigate the pathological role of staphylococcal enterotoxins (SEs) and 
      toxic shock syndrome toxin-1 (TSST-1) in bovine mastitis, the production of SEs 
      and TSST-1 was investigated in staphylococci isolated from 120 mammary gland 
      secretions (MGS, 51 from no clinical sign-mammary glands and 69 from 
      staphylococcal mastitic-mammary glands) collected from dairy farms where 
      staphylococcal mastitis frequently occurred in Miyagi and Yamagata prefectures 
      from 1997 to 1998. Concentrations of these toxins and specific antibody titers in 
      each MGS were also measured. Furthermore, SEC and TSST-1 were inoculated into 
      lactating mammary glands and inflammatory responses were analyzed. A high 
      percentage of staphylococci including Staphylococcus aureus and 
      coagulase-negative staphylococci isolated from both no clinical sign- and 
      mastitic-MGS produced both SEC and/or TSST-1. The concentration of SEC increased 
      with the severity of the mastitis, and was significantly higher (P<0.05) in acute 
      mastitic-than in no clinical signs-MGS. Titers of specific antibodies to TSST-1 
      in MGS were significantly higher (P<0.05) than those to SEC, regardless of 
      whether or not the cows were lactating or mastitic. Specific antibodies purified 
      from MGS neutralized each toxin in vitro. A significant increase (P < 0.05) in 
      somatic cell counts was induced by the intramammary inoculation of SEC but not 
      TSST-1. These findings indicated that SEC rather than TSST-1 plays an important 
      role in the pathology of staphylococcal bovine mastitis. The inflammatory 
      activity of TSST-1 was probably neutralized by specific antibodies in MGS.
FAU - Kuroishi, Toshinobu
AU  - Kuroishi T
AD  - T Cell Research Institute, Minami-yoshinari, Aoba-ku, Sendai, Japan.
FAU - Komine, Ken-ichi
AU  - Komine K
FAU - Kai, Kenzo
AU  - Kai K
FAU - Itagaki, Masashi
AU  - Itagaki M
FAU - Kobayashi, Jin
AU  - Kobayashi J
FAU - Ohta, Minoru
AU  - Ohta M
FAU - Kamata, Shin-ichi
AU  - Kamata S
FAU - Kumagai, Katsuo
AU  - Kumagai K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Superantigens)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - 39424-54-9 (enterotoxin C, staphylococcal)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/*analysis
MH  - Antibody Specificity
MH  - *Bacterial Toxins
MH  - Cattle
MH  - Cattle Diseases/immunology/*microbiology
MH  - Cell Culture Techniques
MH  - Enterotoxins/*immunology/toxicity
MH  - Female
MH  - Lactation
MH  - Mammary Glands, Animal/cytology/immunology/*microbiology
MH  - Staphylococcal Infections/immunology/*veterinary
MH  - Staphylococcus aureus/immunology
MH  - Superantigens/*immunology
EDAT- 2003/09/03 05:00
MHDA- 2003/12/06 05:00
CRDT- 2003/09/03 05:00
PHST- 2003/09/03 05:00 [pubmed]
PHST- 2003/12/06 05:00 [medline]
PHST- 2003/09/03 05:00 [entrez]
AID - 10.1292/jvms.65.899 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2003 Aug;65(8):899-906. doi: 10.1292/jvms.65.899.