PMID- 12953811
OWN - NLM
STAT- MEDLINE
DCOM- 20031020
LR  - 20191026
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 78
IP  - 2
DP  - 2003 Aug
TI  - Fluorescence in situ hybridization detection of chromosome IGH/BCL2 
      translocations from paraffin-embedded tissue: evaluation in follicular lymphoma.
PG  - 154-9
AB  - Follicular lymphoma is categorized as low-grade lymphoma because of the long 
      median survival, but the disease is difficult to cure because of frequent 
      recurrence. The t(14;18)(q32;q21) associated with follicular lymphoma juxtaposes 
      a portion of BCL-2 (18q21) and IGH(14q32); the result is bcl-2 overexpression. In 
      this study, a highly sensitive 2-color fluorescence in situ hybridization (FISH) 
      method was used to detect t(14;18)(q32;q21) in the nuclei of paraffin-embedded 
      tissue sections. Fourteen specimens, including 11 samples from follicular 
      lymphoma and 3 samples from diffuse large cell lymphoma, for which results of 
      karyotype study and paraffin-embedded tissues were available were selected for 
      FISH study. The FISH results were compared with results of karyotype study of the 
      lymph nodes involved in lymphoma. Among our 11 patients with the diagnosis of 
      follicular lymphoma in whom karyotype study was performed, 8 had 
      t(14;18)(q32;q21) in karyotype analysis, and 7 of these patients had a positive 
      pattern in FISH analysis. In 1 case, FISH analysis was difficult because of weak 
      signals. All 3 patients with diffuse large cell lymphoma and t(14;18)(q32;q21) in 
      karyotype analysis had a positive pattern in FISH analysis. In 3 cases of 
      follicular lymphoma without t(14;18)(q32;q21) in karyotype analysis, FISH did not 
      show a positive pattern. Therefore the FISH assay in tissue was found to be very 
      sensitive in detection of IGH/BCL2 translocation and was helpful in diagnosis of 
      follicular lymphoma or in clarification of the cell origin of lymphoma when 
      karyotype analysis was not available. Performing FISH on paraffin sections also 
      is useful because we can identify cells with genetic abnormalities in the tumor 
      and make a retrospective cytogenetic diagnosis even with old paraffin-embedded 
      specimens.
FAU - Hirose, Yuko
AU  - Hirose Y
AD  - Division of Hematology and Immunology, Department of Internal Medicine, Kanazawa 
      Medical University, Ishikawa, Japan. y-hirose@kanazawa-med.ac.jp
FAU - Masaki, Yasufumi
AU  - Masaki Y
FAU - Ozaki, Mamoru
AU  - Ozaki M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Lymphoma, Follicular/*genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics
MH  - *Translocation, Genetic
EDAT- 2003/09/05 05:00
MHDA- 2003/10/21 05:00
CRDT- 2003/09/05 05:00
PHST- 2003/09/05 05:00 [pubmed]
PHST- 2003/10/21 05:00 [medline]
PHST- 2003/09/05 05:00 [entrez]
AID - 10.1007/BF02983385 [doi]
PST - ppublish
SO  - Int J Hematol. 2003 Aug;78(2):154-9. doi: 10.1007/BF02983385.