PMID- 12954717
OWN - NLM
STAT- MEDLINE
DCOM- 20031215
LR  - 20231213
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 130
IP  - 21
DP  - 2003 Nov
TI  - On the topographic targeting of basal vomeronasal axons through Slit-mediated 
      chemorepulsion.
PG  - 5073-82
AB  - The vomeronasal projection conveys information provided by pheromones and 
      detected by neurones in the vomeronasal organ (VNO) to the accessory olfactory 
      bulb (AOB) and thence to other regions of the brain such as the amygdala. The 
      VNO-AOB projection is topographically organised such that axons from apical and 
      basal parts of the VNO terminate in the anterior and posterior AOB respectively. 
      We provide evidence that the Slit family of axon guidance molecules and their 
      Robo receptors contribute to the topographic targeting of basal vomeronasal 
      axons. Robo receptor expression is confined largely to basal VNO axons, while 
      Slits are differentially expressed in the AOB with a higher concentration in the 
      anterior part, which basal axons do not invade. Immunohistochemistry using a 
      Robo-specific antibody reveals a zone-specific targeting of VNO axons in the AOB 
      well before cell bodies of these neurones in the VNO acquire their final zonal 
      position. In vitro assays show that Slit1-Slit3 chemorepel VNO axons, suggesting 
      that basal axons are guided to the posterior AOB due to chemorepulsive activity 
      of Slits in the anterior AOB. These data in combination with recently obtained 
      other data suggest a model for the topographic targeting in the vomeronasal 
      projection where ephrin-As and neuropilins guide apical VNO axons, while 
      Robo/Slit interactions are important components in the targeting of basal VNO 
      axons.
FAU - Knoll, Bernd
AU  - Knoll B
AD  - MRC Centre for Developmental Neurobiology, King's College London, New Hunt's 
      House, Guy's Hospital Campus, London SE1 1UL, UK.
FAU - Schmidt, Hannes
AU  - Schmidt H
FAU - Andrews, William
AU  - Andrews W
FAU - Guthrie, Sarah
AU  - Guthrie S
FAU - Pini, Adrian
AU  - Pini A
FAU - Sundaresan, Vasi
AU  - Sundaresan V
FAU - Drescher, Uwe
AU  - Drescher U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030903
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Ephrins)
RN  - 0 (Glycoproteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Semaphorins)
RN  - 0 (slit protein, vertebrate)
SB  - IM
MH  - Afferent Pathways
MH  - Animals
MH  - Axons/*metabolism
MH  - Chemotaxis/*physiology
MH  - Ephrins/metabolism
MH  - Glycoproteins/genetics/*metabolism
MH  - In Situ Hybridization
MH  - Mice
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Olfactory Bulb/*anatomy & histology/growth & development/metabolism
MH  - Receptors, Immunologic/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Semaphorins/metabolism
MH  - Vomeronasal Organ/*cytology/growth & development/metabolism
MH  - Roundabout Proteins
EDAT- 2003/09/05 05:00
MHDA- 2003/12/16 05:00
CRDT- 2003/09/05 05:00
PHST- 2003/09/05 05:00 [pubmed]
PHST- 2003/12/16 05:00 [medline]
PHST- 2003/09/05 05:00 [entrez]
AID - dev.00726 [pii]
AID - 10.1242/dev.00726 [doi]
PST - ppublish
SO  - Development. 2003 Nov;130(21):5073-82. doi: 10.1242/dev.00726. Epub 2003 Sep 3.