PMID- 12955176
OWN - NLM
STAT- MEDLINE
DCOM- 20040413
LR  - 20210410
IS  - 0342-4642 (Print)
IS  - 0342-4642 (Linking)
VI  - 29
IP  - 12
DP  - 2003 Dec
TI  - Human leukocyte antigen-DR expression in peripheral blood mononuclear cells from 
      healthy donors influenced by the sera of injured patients prone to severe sepsis.
PG  - 2285-2290
LID - 10.1007/s00134-003-1992-8 [doi]
AB  - OBJECTIVE: To study the influence of sera from severely injured patients on the 
      human leukocyte antigen (HLA)-DR expression of normal peripheral blood 
      mononuclear cells (PBMC). DESIGN: In vitro study. SETTING: University hospital. 
      PATIENTS AND PARTICIPANTS: Sera from 34 patients were obtained within 8 h after 
      trauma. Seventeen of these patients developed posttraumatic sepsis (sepsis group) 
      and 17 recovered without infectious complications. Sera from ten healthy 
      individuals served as controls. Phytohemagglutinin (PHA)-activated PBMC from 44 
      healthy donors were used to study the effects of a patient's serum. MEASUREMENTS 
      AND RESULTS: Medium containing 5% of serum from the sepsis group significantly ( 
      p<0.05) reduced the HLA-DR expression (channels, mean +/- standard error of the 
      mean) on monocytes (patients 883+/-22, controls 962+/-15), B (patients 922+/-14, 
      controls 972+/-7) and T cells (patients 932+/-13, controls 968+/-5) of 
      PHA-activated PBMC. Significantly increased accumulation of TNFalpha on 
      (1.8+/-0.4% of PBMC) and within T cells (0.98+/-0.26% of PBMC) was observed by 
      flow cytometry after incubation with medium containing sera of the sepsis group 
      compared with controls (on 0.5+/-0.1%, within 0.27+/-0.05% of PBMC). A 
      significant negative correlation between relative cell counts of intracellular 
      TNFalpha-positive T cells with HLA-DR expression was observed for monocytes ( r= 
      -0.61), B cells ( r= -0.57) and proliferation ( r= -0.68) as estimated by 
      (3)H-thymidine uptake [patients 139971+/-12844 counts per minute (cpm), controls 
      198973+/-19347 cpm, p<0.05] in the presence of sera from the sepsis group. 
      CONCLUSIONS: Reduced cellular immunity and, therefore, immunodeficiency after 
      trauma appears to be caused by soluble factors influencing T cell function in 
      particular.
FAU - Mueller, Anke
AU  - Mueller A
AD  - Institute of Immunology, University of Essen, Virchowstrasse 171, 45122 , Essen, 
      Germany.
FAU - Kreuzfelder, Ernst
AU  - Kreuzfelder E
AD  - Institute of Immunology, University of Essen, Virchowstrasse 171, 45122 , Essen, 
      Germany.
FAU - Nyadu, Baerbel
AU  - Nyadu B
AD  - Institute of Immunology, University of Essen, Virchowstrasse 171, 45122 , Essen, 
      Germany.
FAU - Lindemann, Monika
AU  - Lindemann M
AD  - Institute of Immunology, University of Essen, Virchowstrasse 171, 45122 , Essen, 
      Germany.
FAU - Rebmannn, Vera
AU  - Rebmannn V
AD  - Institute of Immunology, University of Essen, Virchowstrasse 171, 45122 , Essen, 
      Germany.
FAU - Majetschak, Matthias
AU  - Majetschak M
AD  - Department of Trauma Surgery, University Hospital Mannheim, Ruprecht-Karls 
      University, Theodor-Kutzer-Ufer 1-3, 68167 , Mannheim, Germany.
FAU - Obertacke, Udo
AU  - Obertacke U
AD  - Department of Trauma Surgery, University Hospital Mannheim, Ruprecht-Karls 
      University, Theodor-Kutzer-Ufer 1-3, 68167 , Mannheim, Germany.
FAU - Schade, Ulrich F
AU  - Schade UF
AD  - Department of Trauma Surgery, University of Essen, Hufelandstr. 55, 45122 , 
      Essen, Germany.
FAU - Nast-Kolb, Dieter
AU  - Nast-Kolb D
AD  - Department of Trauma Surgery, University of Essen, Hufelandstr. 55, 45122 , 
      Essen, Germany.
FAU - Grosse-Wilde, Hans
AU  - Grosse-Wilde H
AD  - Institute of Immunology, University of Essen, Virchowstrasse 171, 45122 , Essen, 
      Germany. immunologie@uni-essen.de.
LA  - eng
PT  - Journal Article
DEP - 20030904
PL  - United States
TA  - Intensive Care Med
JT  - Intensive care medicine
JID - 7704851
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/*blood/immunology
MH  - Humans
MH  - Injury Severity Score
MH  - Leukocytes, Mononuclear/*immunology
MH  - Male
MH  - Middle Aged
MH  - Sepsis/*blood/immunology
MH  - Wounds and Injuries/*blood/classification
EDAT- 2003/09/05 05:00
MHDA- 2004/04/14 05:00
CRDT- 2003/09/05 05:00
PHST- 2002/08/27 00:00 [received]
PHST- 2003/08/01 00:00 [accepted]
PHST- 2003/09/05 05:00 [pubmed]
PHST- 2004/04/14 05:00 [medline]
PHST- 2003/09/05 05:00 [entrez]
AID - 10.1007/s00134-003-1992-8 [pii]
AID - 10.1007/s00134-003-1992-8 [doi]
PST - ppublish
SO  - Intensive Care Med. 2003 Dec;29(12):2285-2290. doi: 10.1007/s00134-003-1992-8. 
      Epub 2003 Sep 4.