PMID- 12955404
OWN - NLM
STAT- MEDLINE
DCOM- 20040601
LR  - 20131121
IS  - 0300-8428 (Print)
IS  - 0300-8428 (Linking)
VI  - 98
IP  - 5
DP  - 2003 Sep
TI  - Negative effect of nitric oxide on shortening-frequency relationship in cardiac 
      myocytes is diminished after simulated ischemia-reperfusion.
PG  - 311-8
AB  - Increasing stimulation rate increases function in cardiac myocytes and nitric 
      oxide and cyclic GMP inhibit this effect. We tested the hypothesis that myocyte 
      stunning would blunt both the effects of increases in rate and of nitric oxide 
      and cyclic GMP. Ventricular myocytes from 11 rabbits were used to determine 
      maximum rate of shortening (R(max), microm/s) and % shortening during control and 
      after simulated ischemia [15 min 95% N(2)- 5% CO(2)] and reperfusion 
      [reoxygenation]. Measurements were obtained at 1-4 Hz with vehicle, 
      1H[1,2,4]oxadiazolo[4,3,alpha] quinoxaline-1-one (ODQ) 10(-6) M, soluble guanylyl 
      cyclase inhibitor, or N(G)-nitro-L-arginine methyl ester, nitric oxide synthase 
      inhibitor (L-NAME) 10(-5) M. In control, increases in rate increased R(max) from 
      69 +/- 3 to 254+/-12 and % shortening from 5.3 +/- 0.3 to 8.7 +/- 0.5. Both ODQ 
      and L-NAME shifted values higher. With stunning, the effects of pacing on Rmax 
      and % shortening were blunted and ODQ and L-NAME failed to alter these values. 
      Cyclic GMP was 322+/-37 pmol/10(5) myocytes at baseline and these values were 
      lowered by ODQ (244 +/- 31) and LNAME (207 +/- 23), and similar changes were 
      observed in stunned myocytes. Increasing frequency increased function, and 
      reducing nitric oxide/cyclic GMP enhanced this relationship. The effect of nitric 
      oxide was diminished by stunning, but this was not related to altered cyclic GMP 
      levels. This suggested changes in effects of cyclic GMP downstream to its 
      production during myocardial stunning.
FAU - Weiss, Harvey R
AU  - Weiss HR
AD  - Department of Physiology and Biophysics, UMDNJ-Robert Wood Johnson Medical 
      School, 675 Hoes Lane, Piscataway, NJ 08854-5635, USA. hweiss@umdnj.edu
FAU - Gandhi, Ankur
AU  - Gandhi A
FAU - Scholz, Peter M
AU  - Scholz PM
LA  - eng
PT  - Journal Article
DEP - 20030719
PL  - Germany
TA  - Basic Res Cardiol
JT  - Basic research in cardiology
JID - 0360342
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Animals
MH  - Cell Size
MH  - Cyclic GMP/*metabolism
MH  - *Heart Rate
MH  - *Myocardial Reperfusion Injury/pathology/physiopathology
MH  - Myocardial Stunning/etiology/metabolism/pathology/*physiopathology
MH  - Myocytes, Cardiac/*metabolism/pathology
MH  - Nitric Oxide/*metabolism
MH  - Rabbits
EDAT- 2003/09/05 05:00
MHDA- 2004/06/02 05:00
CRDT- 2003/09/05 05:00
PHST- 2002/11/21 00:00 [received]
PHST- 2003/05/19 00:00 [revised]
PHST- 2003/06/03 00:00 [accepted]
PHST- 2003/09/05 05:00 [pubmed]
PHST- 2004/06/02 05:00 [medline]
PHST- 2003/09/05 05:00 [entrez]
AID - 10.1007/s00395-003-0425-8 [doi]
PST - ppublish
SO  - Basic Res Cardiol. 2003 Sep;98(5):311-8. doi: 10.1007/s00395-003-0425-8. Epub 
      2003 Jul 19.