PMID- 12956709
OWN - NLM
STAT- MEDLINE
DCOM- 20031023
LR  - 20220129
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 18
IP  - 5
DP  - 2003 Sep
TI  - Co-localization of brain-derived neurotrophic factor (BDNF) and wild-type 
      huntingtin in normal and quinolinic acid-lesioned rat brain.
PG  - 1093-102
AB  - Loss of huntingtin-mediated brain-derived neurotrophic factor (BDNF) gene 
      transcription has been described in Huntington's disease (HD) [Zuccato et al. 
      (2001) Science, 293, 493-498]. It has been shown that BDNF is synthesized in the 
      pyramidal layer of cerebral cortex and released in the striatum [Altar et al. 
      (1997) Nature, 389, 856-860; Conner et al. (1997) J. Neurosci., 17, 2295-2313]. 
      Here we show the cellular localization of BDNF in huntingtin-containing neurons 
      in normal rat brain; our double-label immunofluorescence study shows that 
      huntingtin and BDNF are co-contained in approximately 99% of pyramidal neurons of 
      motor cortex. In the striatum, huntingtin is expressed in 75% of neurons 
      containing BDNF. In normal striatum we also show that BDNF is contained in 
      cholinergic and in NOS-containing interneurons, which are relatively resistant to 
      HD degeneration. Furthermore, we show a reduction in huntingtin and in BDNF 
      immunoreactivity in cortical neurons after striatal excitotoxic lesion. Our data 
      are confirmed by an ELISA study of BDNF and by a Western blot analysis of 
      huntingtin in cortex of quinolic acid (QUIN)-lesioned hemispheres. In the 
      lesioned striatum we describe that the striatal subpopulation of cholinergic 
      neurons, surviving degeneration, contain BDNF. The finding that BDNF is contained 
      in nearly all neurons that contain huntingtin in the normal cortex, along with 
      the reduced expression of BDNF after QUIN injection of the striatum, shows that 
      huntingtin may be required for BDNF production in cortex.
FAU - Fusco, Francesca R
AU  - Fusco FR
AD  - Basal Ganglia Unit, Laboratory of Experimental Neurorehabilitation, Santa Lucia 
      Foundation IRCCS, Via Ardeatina 306, Rome 00179, Italy. f.fusco@hsantalucia.it
FAU - Zuccato, Chiara
AU  - Zuccato C
FAU - Tartari, Marzia
AU  - Tartari M
FAU - Martorana, Alessandro
AU  - Martorana A
FAU - De March, Zena
AU  - De March Z
FAU - Giampa, Carmela
AU  - Giampa C
FAU - Cattaneo, Elena
AU  - Cattaneo E
FAU - Bernardi, Giorgio
AU  - Bernardi G
LA  - eng
GR  - GGP02215/TI_/Telethon/Italy
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Dopamine and cAMP-Regulated Phosphoprotein 32)
RN  - 0 (HTT protein, human)
RN  - 0 (Htt protein, rat)
RN  - 0 (Huntingtin Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - F6F0HK1URN (Quinolinic Acid)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain Injuries/chemically induced/*metabolism/pathology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Calcium-Binding Proteins/metabolism
MH  - Cell Count
MH  - Choline O-Acetyltransferase/metabolism
MH  - Corpus Striatum/cytology/injuries/metabolism
MH  - Disease Models, Animal
MH  - Dopamine and cAMP-Regulated Phosphoprotein 32
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Functional Laterality
MH  - Humans
MH  - Huntingtin Protein
MH  - Huntington Disease/metabolism/pathology
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Confocal/instrumentation/methods
MH  - Motor Cortex/cytology/injuries/metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/*metabolism
MH  - Nitric Oxide Synthase/metabolism
MH  - Nuclear Proteins/*metabolism
MH  - Phosphoproteins/metabolism
MH  - *Quinolinic Acid
MH  - Rats
MH  - Rats, Wistar
EDAT- 2003/09/06 05:00
MHDA- 2003/10/24 05:00
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2003/10/24 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
AID - 2844 [pii]
AID - 10.1046/j.1460-9568.2003.02844.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2003 Sep;18(5):1093-102. doi: 10.1046/j.1460-9568.2003.02844.x.