PMID- 12956715
OWN - NLM
STAT- MEDLINE
DCOM- 20031023
LR  - 20220316
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 18
IP  - 5
DP  - 2003 Sep
TI  - Release of BDNF and GABA in the dorsal horn of neuropathic rats.
PG  - 1169-74
AB  - Damage to peripheral nerves is associated with changes in excitability and/or 
      phenotype of primary afferent neurons as well as increased neuronal excitability 
      (central sensitization) and reduced inhibitory tone in the dorsal horn. For 
      instance, in dorsal root ganglia (DRG) brain derived neurotrophic factor (BDNF) 
      is down-regulated in small cells whilst de novo expressed in large diameter 
      cells. In the dorsal horn, GABA content is decreased. In this study, in a dorsal 
      horn, 'with dorsal roots attached' preparation obtained from spinal nerve 
      lesioned Wistar rats, stimulation of ipsilateral dorsal roots at either A fibre 
      or A + C fibre strength did not evoke release of BDNF. In separate experiments, 
      activity-induced release of GABA in the isolated dorsal horn of neuropathic rats 
      was significantly reduced compared to release in sham operated rats. GABA release 
      could be significantly restored following topical application of BDNF through the 
      dorsal horn preparation. Finally, neuropathic rats developed thermal and 
      mechanical hypersensitivity and thermal hyperalgesia was reduced by intrathecal 
      injection of BDNF. We concluded that BDNF-induced release of GABA could be a 
      mechanism to explain the antinociceptive action of intrathecal BDNF in 
      neuropathic animals. Furthermore, reduced availability of sensory neuron-derived 
      BDNF might contribute to the reduced GABAergic tone in the dorsal horn of 
      neuropathic rats.
FAU - Lever, Isobel
AU  - Lever I
AD  - Sensory Function, Centre for Neuroscience Research, London WC1, E6BN, UK.
FAU - Cunningham, Joanna
AU  - Cunningham J
FAU - Grist, John
AU  - Grist J
FAU - Yip, Ping K
AU  - Yip PK
FAU - Malcangio, Marzia
AU  - Malcangio M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GABA Antagonists)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 660YQ98I10 (Potassium Chloride)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Electric Stimulation
MH  - Functional Laterality
MH  - GABA Antagonists/pharmacology
MH  - In Vitro Techniques
MH  - Ligation/methods
MH  - Male
MH  - Nerve Fibers, Myelinated/physiology
MH  - Nerve Fibers, Unmyelinated/physiology
MH  - Pain Measurement/drug effects
MH  - *Peripheral Nerve Injuries
MH  - Posterior Horn Cells/*metabolism
MH  - Potassium Chloride/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Reaction Time
MH  - Sensory Thresholds/drug effects
MH  - Time Factors
MH  - gamma-Aminobutyric Acid/*metabolism
EDAT- 2003/09/06 05:00
MHDA- 2003/10/24 05:00
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2003/10/24 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
AID - 2848 [pii]
AID - 10.1046/j.1460-9568.2003.02848.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2003 Sep;18(5):1169-74. doi: 10.1046/j.1460-9568.2003.02848.x.