PMID- 12956750
OWN - NLM
STAT- MEDLINE
DCOM- 20031215
LR  - 20190901
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 33
IP  - 9
DP  - 2003 Sep
TI  - Immunological analysis of allergenic cross-reactivity between peanut and tree 
      nuts.
PG  - 1273-80
AB  - BACKGROUND: Peanut and tree nut allergy is characterized by a high frequency of 
      life-threatening anaphylactic reactions and typically lifelong persistence. 
      Peanut allergy is more common than tree nut allergy, but many subjects develop 
      hypersensitivity to both peanuts and tree nuts. Whether this is due to the 
      presence of cross-reactive allergens remains unknown. OBJECTIVE: The aim of this 
      study was to investigate the presence of allergenic cross-reactivity between 
      peanut and tree nuts. METHODS: Western blotting and ELISA were performed using 
      sera from subjects with or without peanut and tree nut allergy to assess 
      immunoglobulin E (IgE) reactivity to peanut and tree nut extracts. Inhibition 
      ELISA studies were conducted to assess the presence of allergenic 
      cross-reactivity between peanut and tree nuts. RESULTS: Western blot and ELISA 
      results showed IgE reactivity to peanut, almond, Brazil nut, hazelnut and cashew 
      nut for peanut- and tree nut-allergic subject sera. Raw and roasted peanut and 
      tree nut extracts showed similar IgE reactivities. Inhibition ELISA showed that 
      pre-incubation of sera with almond, Brazil nut or hazelnut extracts resulted in a 
      decrease in IgE binding to peanut extract, indicating allergenic 
      cross-reactivity. Pre-incubation of sera with cashew nut extract did not cause 
      any inhibition. CONCLUSION: These results show that multiple peanut and tree nut 
      sensitivities observed in allergic subjects may be due to cross-reactive B cell 
      epitopes present in different peanut and tree nut allergens. The plant taxonomic 
      classification of peanut and tree nuts does not appear to predict allergenic 
      cross-reactivity.
FAU - de Leon, M P
AU  - de Leon MP
AD  - Department of Allergy, The Alfred Hospital, Australia.
FAU - Glaspole, I N
AU  - Glaspole IN
FAU - Drew, A C
AU  - Drew AC
FAU - Rolland, J M
AU  - Rolland JM
FAU - O'Hehir, R E
AU  - O'Hehir RE
FAU - Suphioglu, C
AU  - Suphioglu C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Allergens/*immunology
MH  - Anacardium/immunology
MH  - Arachis/*immunology
MH  - Bertholletia/immunology
MH  - Blotting, Western/methods
MH  - Corylus/immunology
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Male
MH  - Middle Aged
MH  - Nut Hypersensitivity/*immunology
MH  - Nuts/*immunology
MH  - Peanut Hypersensitivity/*immunology
MH  - Plant Structures/immunology
MH  - Prunus/immunology
EDAT- 2003/09/06 05:00
MHDA- 2003/12/16 05:00
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2003/12/16 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
AID - 1761 [pii]
AID - 10.1046/j.1365-2222.2003.01761.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2003 Sep;33(9):1273-80. doi: 10.1046/j.1365-2222.2003.01761.x.