PMID- 12957655
OWN - NLM
STAT- MEDLINE
DCOM- 20040512
LR  - 20190827
IS  - 0891-5849 (Print)
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 35
IP  - 6
DP  - 2003 Sep 15
TI  - Effects of age and caloric restriction on glutathione redox state in mice.
PG  - 626-35
AB  - The main purpose of this study was to determine whether the aging process in the 
      mouse is associated with a pro-oxidizing shift in the redox state of glutathione 
      and whether restriction of caloric intake, which results in the extension of life 
      span, retards such a shift. Amounts of reduced and oxidized forms of glutathione 
      (GSH and GSSG, respectively) and protein-glutathione mixed disulfides 
      (protein-SSG) were measured in homogenates and mitochondria of liver, kidney, 
      heart, brain, eye, and testis of 4, 10, 22, and 26 month old ad libitum-fed (AL) 
      mice and 22 month old mice fed a diet containing 40% fewer calories than the AL 
      group from the age of 4 months. The concentrations of GSH, GSSG, and protein-SSG 
      vary greatly (approximately 10-, 30-, and 9-fold, respectively) from one tissue 
      to another. During aging, the ratios of GSH:GSSG in mitochondria and tissue 
      homogenates decreased, primarily due to elevations in GSSG content, while the 
      protein-SSG content increased significantly. Glutathione redox potential in 
      mitochondria became less negative, i.e., more pro-oxidizing, as the animal aged. 
      Caloric restriction (CR) lowered the GSSG and protein-SSG content. Results 
      suggest that the aging process in the mouse is associated with a gradual 
      pro-oxidizing shift in the glutathione redox state and that CR attenuates this 
      shift.
FAU - Rebrin, Igor
AU  - Rebrin I
AD  - Department of Molecular Pharmacology and Toxicology, University of Southern 
      California, Los Angeles, CA 90033, USA.
FAU - Kamzalov, Sergey
AU  - Kamzalov S
FAU - Sohal, Rajindar S
AU  - Sohal RS
LA  - eng
GR  - R01 AG013563/AG/NIA NIH HHS/United States
GR  - R01 AG013563-13/AG/NIA NIH HHS/United States
GR  - R01 AG13563/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - GAN16C9B8O (Glutathione)
RN  - ULW86O013H (Glutathione Disulfide)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Brain/metabolism
MH  - *Caloric Restriction
MH  - Eye/metabolism
MH  - Glutathione/*metabolism
MH  - Glutathione Disulfide/metabolism
MH  - Kidney/metabolism
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Mitochondria/metabolism
MH  - Myocardium/metabolism
MH  - Oxidation-Reduction
MH  - Testis/metabolism
PMC - PMC2837076
MID - NIHMS182388
EDAT- 2003/09/06 05:00
MHDA- 2004/05/13 05:00
PMCR- 2010/03/11
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2004/05/13 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
PHST- 2010/03/11 00:00 [pmc-release]
AID - S0891584903003885 [pii]
AID - 10.1016/s0891-5849(03)00388-5 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2003 Sep 15;35(6):626-35. doi: 
      10.1016/s0891-5849(03)00388-5.