PMID- 12957845
OWN - NLM
STAT- MEDLINE
DCOM- 20031024
LR  - 20190901
IS  - 1093-9946 (Print)
IS  - 1093-4715 (Linking)
VI  - 8
DP  - 2003 Sep 1
TI  - Identification, rare-event detection and analysis of dendritic cell subsets in 
      broncho-alveolar lavage fluid and peripheral blood by flow cytometry.
PG  - s1175-80
AB  - Dendritic cells (DCs) and their precursors play important roles, not only as 
      antigen presenting cells, but components of the immunoregulatory network. 
      Depending on their lineage, activation and differentiation state, DCs can promote 
      a strong T-cell response or a state of anergy, and can polarize the T helper 
      response in the direction of TH1 or TH2. The lung comprises one of the major 
      interfaces between the host and the environment and regularly comes into contact 
      with antigens, allergens and pathogens. Inflammation in response to these stimuli 
      must be very carefully regulated in the lung since perturbations, especially of a 
      chronic nature, can result in immunopathology that interferes with the lungs' 
      critical function of gas exchange. Especially in disease states such as chronic 
      obstructive pulmonary disease, which has been associated with a chronic TH1 
      response, and asthma, which is TH2-driven, the ability to access and characterize 
      DCs and their precursors is critical to the understanding of immune modulation in 
      these processes. In this report we will demonstrate that mature DCs and their 
      monocytoid and plasmacytoid precursors can be sampled in the lung by the minimal 
      invasive procedure of broncho-alveolar lavage (BAL) despite their relative 
      scarcity and can be detected by rare event multiparameter flow cytometry.
FAU - Donnenberg, Vera S
AU  - Donnenberg VS
AD  - University of Pittsburgh Cancer Institute, Department of Surgery, The Hillman 
      Cancer Center, 5117 Centre Avenue, Pittsburgh PA 15213, USA. 
      donnenbergad@msx.upmc.edu
FAU - Donnenberg, Albert D
AU  - Donnenberg AD
LA  - eng
GR  - N0I AR9 2239/AR/NIAMS NIH HHS/United States
GR  - P0I AI43664/AI/NIAID NIH HHS/United States
GR  - R01 AI4870/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030901
PL  - United States
TA  - Front Biosci
JT  - Frontiers in bioscience : a journal and virtual library
JID - 9709506
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Antigens, CD/analysis/immunology
MH  - Bronchoalveolar Lavage Fluid/*cytology/immunology
MH  - Cell Separation
MH  - Dendritic Cells/chemistry/*cytology/*immunology/physiology
MH  - Flow Cytometry/methods
MH  - Hematopoietic Stem Cells/chemistry/cytology/immunology/physiology
MH  - Humans
MH  - Immunophenotyping
MH  - Multipotent Stem Cells/chemistry/cytology/immunology/physiology
EDAT- 2003/09/06 05:00
MHDA- 2003/10/25 05:00
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2003/10/25 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
AID - 10.2741/1185 [doi]
PST - epublish
SO  - Front Biosci. 2003 Sep 1;8:s1175-80. doi: 10.2741/1185.