PMID- 12957923
OWN - NLM
STAT- MEDLINE
DCOM- 20040112
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 69
IP  - 9
DP  - 2003 Sep
TI  - Characterization of microbial communities in gas industry pipelines.
PG  - 5354-63
AB  - Culture-independent techniques, denaturing gradient gel electrophoresis (DGGE) 
      analysis, and random cloning of 16S rRNA gene sequences amplified from community 
      DNA were used to determine the diversity of microbial communities in gas industry 
      pipelines. Samples obtained from natural gas pipelines were used directly for DNA 
      extraction, inoculated into sulfate-reducing bacterium medium, or used to 
      inoculate a reactor that simulated a natural gas pipeline environment. The 
      variable V2-V3 (average size, 384 bp) and V3-V6 (average size, 648 bp) regions of 
      bacterial and archaeal 16S rRNA genes, respectively, were amplified from genomic 
      DNA isolated from nine natural gas pipeline samples and analyzed. A total of 106 
      bacterial 16S rDNA sequences were derived from DGGE bands, and these formed three 
      major clusters: beta and gamma subdivisions of Proteobacteria and gram-positive 
      bacteria. The most frequently encountered bacterial species was Comamonas 
      denitrificans, which was not previously reported to be associated with microbial 
      communities found in gas pipelines or with microbially influenced corrosion. The 
      31 archaeal 16S rDNA sequences obtained in this study were all related to those 
      of methanogens and phylogenetically fall into three clusters: order I, 
      Methanobacteriales; order III, Methanomicrobiales; and order IV, 
      Methanosarcinales: Further microbial ecology studies are needed to better 
      understand the relationship among bacterial and archaeal groups and the 
      involvement of these groups in the process of microbially influenced corrosion in 
      order to develop improved ways of monitoring and controlling microbially 
      influenced corrosion.
FAU - Zhu, Xiang Y
AU  - Zhu XY
AD  - Environmental Science and Technology Center, Gas Technology Institute, Des 
      Plaines, Illinois 60018, USA. xiangyang.zhu@gastechnology.org
FAU - Lubeck, John
AU  - Lubeck J
FAU - Kilbane, John J 2nd
AU  - Kilbane JJ 2nd
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Gasoline)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*classification/genetics/*isolation & purification/ultrastructure
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - DNA, Ribosomal/genetics
MH  - *Gasoline
MH  - Gene Amplification
MH  - Microscopy, Electron, Scanning
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/*genetics
PMC - PMC194955
EDAT- 2003/09/06 05:00
MHDA- 2004/01/13 05:00
PMCR- 2003/09/01
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2004/01/13 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
PHST- 2003/09/01 00:00 [pmc-release]
AID - 0416 [pii]
AID - 10.1128/AEM.69.9.5354-5363.2003 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2003 Sep;69(9):5354-63. doi: 
      10.1128/AEM.69.9.5354-5363.2003.