PMID- 12957960
OWN - NLM
STAT- MEDLINE
DCOM- 20040112
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 69
IP  - 9
DP  - 2003 Sep
TI  - Use of DNA and peptide nucleic acid molecular beacons for detection and 
      quantification of rRNA in solution and in whole cells.
PG  - 5673-8
AB  - DNA and peptide nucleic acid (PNA) molecular beacons were successfully used to 
      detect rRNA in solution. In addition, PNA molecular beacon hybridizations were 
      found to be useful for the quantification of rRNA: hybridization signals 
      increased in a linear fashion with the 16S rRNA concentrations used in this 
      experiment (between 0.39 and 25 nM) in the presence of 50 nM PNA MB. DNA and PNA 
      molecular beacons were successfully used to detect whole cells in fluorescence in 
      situ hybridization (FISH) experiments without a wash step. The FISH results with 
      the PNA molecular beacons were superior to those with the DNA molecular beacons: 
      the hybridization kinetics were much faster, the signal-to-noise ratio was much 
      higher, and the specificity was much better for the PNA molecular beacons. 
      Finally, it was demonstrated that the combination of the use of PNA molecular 
      beacons in FISH and flow cytometry makes it possible to rapidly collect 
      quantitative FISH data. Thus, PNA molecular beacons might provide a solution for 
      limitations of traditional FISH methods, such as variable target site 
      accessibility, poor sensitivity for target cells with low rRNA content, 
      background fluorescence, and applications of FISH in microfluidic devices.
FAU - Xi, Chuanwu
AU  - Xi C
AD  - Department of Civil and Environmental Engineering, University of Illinois at 
      Urbana-Champaign, Urbana, Illinois 61801, USA.
FAU - Balberg, Michal
AU  - Balberg M
FAU - Boppart, Stephen A
AU  - Boppart SA
FAU - Raskin, Lutgarde
AU  - Raskin L
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Biomarkers)
RN  - 0 (DNA Primers)
RN  - 0 (Peptide Nucleic Acids)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Solutions)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - Biomarkers
MH  - *DNA
MH  - DNA Primers
MH  - Escherichia coli/*genetics
MH  - Flow Cytometry/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - *Peptide Nucleic Acids
MH  - RNA, Ribosomal/*analysis
MH  - RNA, Ribosomal, 16S/*analysis/genetics
MH  - Solutions
PMC - PMC194960
EDAT- 2003/09/06 05:00
MHDA- 2004/01/13 05:00
PMCR- 2003/09/01
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2004/01/13 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
PHST- 2003/09/01 00:00 [pmc-release]
AID - 0435 [pii]
AID - 10.1128/AEM.69.9.5673-5678.2003 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2003 Sep;69(9):5673-8. doi: 
      10.1128/AEM.69.9.5673-5678.2003.