PMID- 12958055
OWN - NLM
STAT- MEDLINE
DCOM- 20040212
LR  - 20141120
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 168
IP  - 12
DP  - 2003 Dec 15
TI  - Modulation of bacterial growth by tumor necrosis factor-alpha in vitro and in 
      vivo.
PG  - 1462-70
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays an important role in innate 
      immunity. Recent in vitro studies have shown that TNF-alpha may also serve as a 
      growth factor for some bacteria. We examined the physiologic relevance of this 
      phenomenon both in vitro and in vivo. Recombinant mouse TNF-alpha increased in 
      vitro proliferation of Escherichia coli but not Pseudomonas aeruginosa in a 
      concentration-dependent manner, and this effect was attenuated by anti-TNF-alpha 
      antibodies. However, in vivo, TNF-alpha gene-deficient (TNF-alpha-/-) mice showed 
      higher mortality than wild-type (TNF-alpha+/+) mice after inoculation of 
      intranasal bacteria. An impaired bacterial clearance in TNF-alpha-/- mice was 
      associated with decreased systemic concentrations of chemokine macrophage 
      inflammatory protein-2, reduced pulmonary neutrophil recruitment, and depressed 
      expression of neutrophil CD11b and CD16/CD32, suggesting that the effect of 
      TNF-alpha on E. coli growth was outweighed by the recruited neutrophils. We also 
      demonstrated that neutropenic TNF-alpha+/+ mice had approximately 100-fold higher 
      E. coli counts in their lungs than TNF-alpha-/- mice, although survival rates in 
      both groups were similar. We conclude that TNF-alpha augments E. coli growth in 
      vitro and in vivo. However, in vivo, this effect becomes only apparent in 
      neutropenic animals. The relevance of these findings for immune compromised 
      patients remains to be investigated.
FAU - Lee, Jin-Hwa
AU  - Lee JH
AD  - Department of Anaesthesia, St. Michael's Hospital, Toronto, ON, M5B 1W8 Canada.
FAU - Del Sorbo, Lorenzo
AU  - Del Sorbo L
FAU - Khine, Aye Aye
AU  - Khine AA
FAU - de Azavedo, Joyce
AU  - de Azavedo J
FAU - Low, Donald E
AU  - Low DE
FAU - Bell, David
AU  - Bell D
FAU - Uhlig, Stefan
AU  - Uhlig S
FAU - Slutsky, Arthur S
AU  - Slutsky AS
FAU - Zhang, Haibo
AU  - Zhang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030904
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
CIN - Am J Respir Crit Care Med. 2003 Dec 15;168(12):1410-1. PMID: 14668252
MH  - Animals
MH  - Escherichia coli/drug effects/*growth & development
MH  - Immunocompromised Host/drug effects/*immunology
MH  - In Vitro Techniques
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Neutropenia/immunology
MH  - Neutrophil Activation/drug effects/immunology
MH  - Neutrophil Infiltration/drug effects/immunology
MH  - Pneumonia, Bacterial/*immunology/microbiology
MH  - Pseudomonas aeruginosa/drug effects/*growth & development
MH  - Tumor Necrosis Factor-alpha/genetics/*immunology/pharmacology
EDAT- 2003/09/06 05:00
MHDA- 2004/02/13 05:00
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2004/02/13 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
AID - 200302-303OC [pii]
AID - 10.1164/rccm.200302-303OC [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2003 Dec 15;168(12):1462-70. doi: 
      10.1164/rccm.200302-303OC. Epub 2003 Sep 4.