PMID- 12958264
OWN - NLM
STAT- MEDLINE
DCOM- 20031118
LR  - 20220331
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 41
IP  - 9
DP  - 2003 Sep
TI  - Evaluation of three rapid methods for the direct identification of Staphylococcus 
      aureus from positive blood cultures.
PG  - 4324-7
AB  - Staphylococci represent the most commonly encountered blood culture isolates. 
      Differentiating Staphylococcus aureus from coagulase-negative staphylococci 
      (CoNS) is important in guiding empirical therapy, especially since the majority 
      of CoNS are contaminants. This study evaluated three rapid methods for the direct 
      identification of S. aureus from blood cultures. A total of 157 patient blood 
      cultures with gram stains showing gram-positive cocci in clusters were included. 
      The following assays were evaluated: API RAPIDEC staph (API) (bioMerieux, Durham, 
      N.C.), the tube coagulase test (TCT) read at 4 h, and peptide nucleic acid (PNA) 
      fluorescence in situ hybridization (FISH) (AdvanDx, Woburn, Mass.). All assays 
      yielded results of S. aureus or non-S. aureus. The direct rapid results were 
      compared to results obtained with isolated colonies using the AccuProbe 
      Staphylococcus aureus Culture Identification Test (Gen-Probe, San Diego, Calif.). 
      API, TCT, and PNA FISH exhibited sensitivities of 96, 84, and 99% and 
      specificities of 99, 100, and 100%, respectively. Direct identification testing 
      by any of these three assays yielded acceptable performance and timely results. 
      This ability to accurately detect S. aureus in blood cultures gives the physician 
      information with which to initiate or tailor antimicrobial therapy. Coupled with 
      direct susceptibility testing of positive blood culture broths, the patient and 
      institution may experience improved outcomes.
FAU - Chapin, Kimberle
AU  - Chapin K
AD  - Lahey Clinic, Burlington, Massachusetts 01805, USA. Kimberle_c_chapin@lahey.org
FAU - Musgnug, Michael
AU  - Musgnug M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Coagulase)
RN  - 0 (Peptide Nucleic Acids)
SB  - IM
MH  - Bacteremia/*diagnosis
MH  - Bacteriological Techniques/*methods
MH  - Coagulase/analysis
MH  - Costs and Cost Analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Peptide Nucleic Acids/analysis
MH  - Staphylococcus aureus/*isolation & purification
PMC - PMC193828
EDAT- 2003/09/06 05:00
MHDA- 2003/12/03 05:00
PMCR- 2003/09/01
CRDT- 2003/09/06 05:00
PHST- 2003/09/06 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/09/06 05:00 [entrez]
PHST- 2003/09/01 00:00 [pmc-release]
AID - 0553 [pii]
AID - 10.1128/JCM.41.9.4324-4327.2003 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2003 Sep;41(9):4324-7. doi: 10.1128/JCM.41.9.4324-4327.2003.