PMID- 12960034
OWN - NLM
STAT- MEDLINE
DCOM- 20031203
LR  - 20091119
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 144
IP  - 11
DP  - 2003 Nov
TI  - Estrogen enhances retrograde transport of brain-derived neurotrophic factor in 
      the rodent forebrain.
PG  - 5022-9
AB  - The neurotrophins and their receptors activate signaling molecules to regulate 
      neural function in development and adulthood. Neurons in the septum-diagonal band 
      complex (or basal forebrain) derive neurotrophins through retrograde transport of 
      these peptides from their forebrain targets. The present study tests the 
      hypothesis that the gonadal hormone estrogen enhances retrograde transport of the 
      neurotrophin brain-derived neurotrophic factor (BDNF). Estrogen increases BDNF 
      expression in the horizontal limb of the diagonal band of Broca (hlDBB) and its 
      forebrain target the olfactory bulb. In the present study, rhodamine-labeled 
      (Rho-) BDNF injected into the olfactory bulb was rapidly transferred to neurons 
      in the hlDBB. Significantly greater numbers of hlDBB neurons were retrogradely 
      labeled with Rho-BDNF in animals pretreated with estrogen, compared with 
      placebo-replaced controls. Anti-tyrosine kinase (trk) B antibodies injected into 
      the olfactory bulb attenuated retrograde transport of Rho-BDNF in a 
      dose-dependent manner, suggesting that estrogen may enhance BDNF transport in 
      this circuit through regulation of its trk receptor. Anti-trkB antibodies also 
      reduced cAMP response element binding protein phosphorylation in the hlDBB and 
      combined injections of anti-trkA and trkB in the olfactory bulb reduced 
      estrogen-induced increases in basal forebrain choline acetyltransferase. These 
      studies support the hypothesis that estrogen facilitates neurotrophin transport 
      in forebrain circuits.
FAU - Jezierski, Melinda K
AU  - Jezierski MK
AD  - Department of Human Anatomy and Medical Neurobiology, Texas A&M University System 
      Health Science Center, College Station, Texas 77843, USA.
FAU - Sohrabji, Farida
AU  - Sohrabji F
LA  - eng
GR  - AG19515/AG/NIA NIH HHS/United States
GR  - NS36297/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030731
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Drug Combinations)
RN  - 0 (Estrogens)
RN  - 0 (Rhodamines)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Antibodies/administration & dosage
MH  - Biological Transport, Active/drug effects
MH  - Brain-Derived Neurotrophic Factor/*pharmacokinetics
MH  - Choline O-Acetyltransferase/antagonists & inhibitors
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Diagonal Band of Broca/cytology/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Drug Combinations
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Neurons/metabolism
MH  - Olfactory Bulb/metabolism
MH  - Phosphorylation/drug effects
MH  - Prosencephalon/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkA/immunology
MH  - Receptor, trkB/immunology
MH  - Rhodamines
EDAT- 2003/09/10 05:00
MHDA- 2003/12/04 05:00
CRDT- 2003/09/10 05:00
PHST- 2003/09/10 05:00 [pubmed]
PHST- 2003/12/04 05:00 [medline]
PHST- 2003/09/10 05:00 [entrez]
AID - en.2003-0724 [pii]
AID - 10.1210/en.2003-0724 [doi]
PST - ppublish
SO  - Endocrinology. 2003 Nov;144(11):5022-9. doi: 10.1210/en.2003-0724. Epub 2003 Jul 
      31.