PMID- 12960246
OWN - NLM
STAT- MEDLINE
DCOM- 20031118
LR  - 20231213
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 74
IP  - 4
DP  - 2003 Oct
TI  - Predominance of Th2-promoting dendritic cells in early human pregnancy decidua.
PG  - 514-22
AB  - Dendritic cells (DCs) are specialized antigen-presenting cells required for the 
      priming and activation of T cells and promote the differentiation of naive CD4+ T 
      cells toward the T helper cell type 1 (Th1) or Th2 phenotype. Here, we describe 
      the characterization of CD45+CD3-CD14-CD16-CD19-CD20-CD56-HLA-DRbright DCs from 
      early human pregnancy decidua by flow cytometry. The percentage of DCs to 
      mononuclear cells (leukocytes) in the decidua was significantly higher than that 
      in the peripheral blood. Moreover, decidual DCs expressed costimulatory molecules 
      such as CD80 and CD86 and a mature marker such as CD83 on their surface. The 
      percentage of CD11c+CD123- myeloid DCs in the decidua was significantly higher 
      than that in the peripheral blood. Conversely, the ratio of CD11c-CD123+ lymphoid 
      DCs in the decidua was significantly lower than that in the peripheral blood. The 
      number of interleukin (IL)-12-producing cells in the total DC population and the 
      myeloid DCs in the decidua was significantly lower than that in the peripheral 
      blood. IL-12 secretion by activated decidual myeloid DCs was significantly lower 
      than that by peripheral DCs. Naive CD4+ T cells primed with decidual myeloid DCs 
      led to a higher percentage of Th2 cells in comparison with that with peripheral 
      myeloid DCs. This finding was abolished by exogenous IL-12 administration with 
      decidual myeloid DCs. Thus, the DCs in the decidua could regulate the Th1/Th2 
      balance to maintain a Th2-dominant state, leading to maintenance of pregnancy.
FAU - Miyazaki, Satomi
AU  - Miyazaki S
AD  - Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical 
      University, Japan.
FAU - Tsuda, Hiroshi
AU  - Tsuda H
FAU - Sakai, Masatoshi
AU  - Sakai M
FAU - Hori, Shinichi
AU  - Hori S
FAU - Sasaki, Yasushi
AU  - Sasaki Y
FAU - Futatani, Takeshi
AU  - Futatani T
FAU - Miyawaki, Toshio
AU  - Miyawaki T
FAU - Saito, Shigeru
AU  - Saito S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030715
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antigens, CD)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Protein Subunits)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Adult
MH  - Antigens, CD
MH  - Decidua/*immunology
MH  - Dendritic Cells/*physiology
MH  - Female
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Immunoglobulins/analysis
MH  - Immunophenotyping
MH  - Interleukin-12/biosynthesis
MH  - Membrane Glycoproteins/analysis
MH  - Pregnancy
MH  - Protein Subunits/biosynthesis
MH  - Th1 Cells/physiology
MH  - Th2 Cells/*physiology
MH  - CD83 Antigen
EDAT- 2003/09/10 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/09/10 05:00
PHST- 2003/09/10 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/09/10 05:00 [entrez]
AID - jlb.1102566 [pii]
AID - 10.1189/jlb.1102566 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2003 Oct;74(4):514-22. doi: 10.1189/jlb.1102566. Epub 2003 Jul 15.