PMID- 12960417
OWN - NLM
STAT- MEDLINE
DCOM- 20040609
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 18
IP  - 5
DP  - 2003 Sep
TI  - Detection by dual color fluorescence in situ hybridization of in vivo chromosome 
      damage in rat hepatocyte nuclei.
PG  - 471-5
AB  - Our previous study using a multicolor fluorescence in situ hybridization (FISH) 
      technique revealed that region-specific DNA probes for rat chromosome 1 enabled 
      the detection of structural chromosome damage in rat interphase nuclei from 
      peripheral blood and bone marrow cells. In the present study, this FISH technique 
      was modified for application to a non-hematopoietic organ (liver) and the 
      usefulness of the system was tested using diethylnitrosamine (DEN) as a model 
      hepatocarcinogen. Male Sprague-Dawley rats were orally treated once with DEN at 
      200 mg/kg. Their livers were removed at 4, 7 or 14 days after treatment and 
      homogenized with a tissue grinder to isolate hepatocyte nuclei. The nucleus 
      suspension was fixed in methanol:acetic acid and air dried. Dual color FISH with 
      two probes, one labeled with tetramethylrhodamine and one with digoxigenin, 
      demonstrated that the maximum increase in the frequency of nuclei with spatially 
      abnormal signals was observed 7 days after treatment. A dose-response 
      relationship for induction of abnormal nuclei was observed. This improved dual 
      color FISH system is potentially valuable for assessing in vivo clastogenicity in 
      all rat organs.
FAU - Matsumoto, Kyomu
AU  - Matsumoto K
AD  - Laboratory of Genetic Toxicology, The Institute of Environmental Toxicology, 4321 
      Uchimoriya-machi, Mitsukaido-shi, Ibaraki 303-0043, Japan. matsumoto@iet.or.jp
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Rhodamines)
RN  - 3IQ78TTX1A (Diethylnitrosamine)
RN  - 62669-72-1 (tetramethylrhodamine)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/drug effects/*genetics
MH  - Chromosomes/drug effects
MH  - DNA Damage/genetics
MH  - DNA Probes/chemistry
MH  - Diethylnitrosamine/toxicity
MH  - Fluorescent Dyes/chemistry
MH  - Hepatocytes/drug effects/*physiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rhodamines/chemistry
EDAT- 2003/09/10 05:00
MHDA- 2004/06/21 10:00
CRDT- 2003/09/10 05:00
PHST- 2003/09/10 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2003/09/10 05:00 [entrez]
AID - 10.1093/mutage/geg023 [doi]
PST - ppublish
SO  - Mutagenesis. 2003 Sep;18(5):471-5. doi: 10.1093/mutage/geg023.