PMID- 12960768
OWN - NLM
STAT- MEDLINE
DCOM- 20031120
LR  - 20190718
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 14
IP  - 11
DP  - 2003 Aug 6
TI  - Sustained intracellular Ca2+ elevation induced by a brief BDNF application in rat 
      visual cortex neurons.
PG  - 1481-3
AB  - A 1-2 min application of brain-derived neurotrophic factor (BDNF; 20 ng/ml) 
      induced sustained elevation of intracellular Ca2+ lasting > 90 min, using the 
      fura-2 imaging of intracellular Ca2+ mobilization, in visual cortical pyramidal 
      neurons isolated from rats. BDNF increased intracellular Ca2+ through the 
      PLC-gamma phosphorylation after the TrkB receptor tyrosine kinase activation. 
      Either K252a or U73122 suppressed intracellular Ca2+ in the absence of BDNF. We 
      suggest that sustained activation of Trk B receptor tyrosine kinase and PLC-gamma 
      occurs after a brief BDNF application and contributes to the short-term 
      maintenance (< 30 min) of the sustained intracellular Ca2+ elevation.
FAU - Mizoguchi, Yoshito
AU  - Mizoguchi Y
AD  - Cellular and Systems Physiology, Graduate School of Medical Sciences, Kyushu 
      University, Fukuoka 812-8582, Japan.
FAU - Nabekura, Junichi
AU  - Nabekura J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Estrenes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indole Alkaloids)
RN  - 0 (Pyrrolidinones)
RN  - 112648-68-7 
      (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Calcium/*metabolism
MH  - Carbazoles/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Estrenes/pharmacology
MH  - Fluorescent Dyes
MH  - Fura-2
MH  - In Vitro Techniques
MH  - Indole Alkaloids
MH  - Phospholipase C gamma
MH  - Phosphorylation
MH  - Pyramidal Cells/drug effects/*metabolism
MH  - Pyrrolidinones/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/antagonists & inhibitors/drug effects/metabolism
MH  - Stimulation, Chemical
MH  - Type C Phospholipases/antagonists & inhibitors/metabolism
MH  - Visual Cortex/cytology/drug effects/*metabolism
EDAT- 2003/09/10 05:00
MHDA- 2003/12/03 05:00
CRDT- 2003/09/10 05:00
PHST- 2003/09/10 05:00 [pubmed]
PHST- 2003/12/03 05:00 [medline]
PHST- 2003/09/10 05:00 [entrez]
AID - 10.1097/00001756-200308060-00015 [doi]
PST - ppublish
SO  - Neuroreport. 2003 Aug 6;14(11):1481-3. doi: 10.1097/00001756-200308060-00015.