PMID- 12962598
OWN - NLM
STAT- MEDLINE
DCOM- 20031002
LR  - 20190513
IS  - 1195-1982 (Print)
IS  - 1195-1982 (Linking)
VI  - 9
IP  - 5
DP  - 2002 Sep-Oct
TI  - Intestinal immunoglobulin a response to naturally acquired enterotoxigenic 
      Escherichia coli in US travelers to an endemic area of Mexico.
PG  - 247-50
AB  - BACKGROUND: Simple methods for detecting secretory immunoglobulin A (sIgA) immune 
      responses following natural enteric infection and oral immunization are needed. 
      METHODS: Fourteen students from the United States acquiring enterotoxigenic 
      Escherichia coli (ETEC) diarrhea in Mexico were studied for fecal immunoglobulin 
      A (IgA) response to their homologous infecting ETEC and to heat-labile (LT) toxin 
      of ETEC using Dot-Blot microfiltration and enzyme-linked immunosorbent assay 
      (ELISA) methods. Paired stool samples were collected on the day of presentation 
      and 5 days later. RESULTS: Twelve of 14 (86%) patients with ETEC diarrhea (5 
      heat-stable [ST]/LT positive, 4 LT-only, and 5 ST-only) developed sIgA antibodies 
      directed against their homologous ETEC and 6 (66%) of the 9 patients harboring 
      ST/LT or LT-only strains developed sIgA LT-antibody responses. Single fecal 
      samples from 9 healthy controls were negative for ETEC specific antibodies. 
      CONCLUSIONS: Patients with diarrhea due to noninvasive ST/LT ETEC and LT ETEC 
      commonly produce a specific sIgA antibody response early in the illness. We feel 
      that the methods employed will be useful to detect antibodies during natural 
      infection by enteric pathogens and following oral enteric vaccine administration.
FAU - Estrada-Garcia, M T
AU  - Estrada-Garcia MT
AD  - Center of Infectious Diseases, The University of Texas School of Public Health, 
      Houston, Texas, USA.
FAU - Jiang, Z-D
AU  - Jiang ZD
FAU - Adachi, J
AU  - Adachi J
FAU - Mathewson, J J
AU  - Mathewson JJ
FAU - DuPont, H L
AU  - DuPont HL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Travel Med
JT  - Journal of travel medicine
JID - 9434456
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Immunoglobulin A)
SB  - IM
MH  - Antibody Formation
MH  - Antigens, Bacterial/immunology
MH  - Diarrhea/*immunology/microbiology
MH  - Enterotoxins/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Escherichia coli Infections/*immunology
MH  - Humans
MH  - Immunoglobulin A/*immunology
MH  - Mexico/epidemiology
MH  - *Travel
MH  - United States
EDAT- 2003/09/10 05:00
MHDA- 2003/10/03 05:00
CRDT- 2003/09/10 05:00
PHST- 2003/09/10 05:00 [pubmed]
PHST- 2003/10/03 05:00 [medline]
PHST- 2003/09/10 05:00 [entrez]
AID - 10.2310/7060.2002.24210 [doi]
PST - ppublish
SO  - J Travel Med. 2002 Sep-Oct;9(5):247-50. doi: 10.2310/7060.2002.24210.