PMID- 12962645
OWN - NLM
STAT- MEDLINE
DCOM- 20040204
LR  - 20151119
IS  - 0141-1136 (Print)
IS  - 0141-1136 (Linking)
VI  - 57
IP  - 1-2
DP  - 2004 Feb-Mar
TI  - Hepatic CYP1A levels and EROD activity in English sole: biomonitoring of marine 
      contaminants in Vancouver Harbour.
PG  - 37-54
AB  - To assess chemical contaminant stress in the marine environment, 
      ethoxyresorufin-O-deethylase (EROD) activity and cytochrome P450 1A (CYP1A) 
      expression were measured in 88 English Sole (Pleuronectes vetulus) collected 
      during May and June 1999 from four sites in Vancouver Harbour and at an expected 
      reference site outside the harbour. Hepatic microsomes were prepared from the 
      fish and analyzed for total CYP content, EROD activity, and CYP1A protein levels. 
      Hepatic EROD activity and CYP1A protein levels were elevated in fish from two 
      sites in the inner harbour. A comparison with sediment chemistry data showed that 
      fish with increased EROD activity and CYP1A levels came from sites containing 
      relatively high levels of polycyclic aromatic hydrocarbons and polychlorinated 
      biphenyls. Unexpectedly high levels of EROD activity and CYP1A protein were also 
      found in fish from a reference site near Gibsons, in Howe Sound. The elevated 
      EROD activity and CYP1A expression in fish from this site cannot be explained by 
      the chemical analysis data collected.
FAU - Miller, K A
AU  - Miller KA
AD  - Faculty of Pharmaceutical Sciences, University of British Columbia, 2146 East 
      Mall, Vancouver, British Columbia, Canada V6T 1Z3.
FAU - Addison, R F
AU  - Addison RF
FAU - Bandiera, S M
AU  - Bandiera SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mar Environ Res
JT  - Marine environmental research
JID - 9882895
RN  - 0 (Biomarkers)
RN  - 0 (Hydrocarbons)
RN  - 0 (Petroleum)
RN  - 0 (Water Pollutants)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Biomarkers/analysis
MH  - Cytochrome P-450 CYP1A1/*pharmacology
MH  - Environmental Monitoring/methods
MH  - Flounder/*physiology
MH  - Geologic Sediments/chemistry
MH  - Hydrocarbons/*poisoning
MH  - Petroleum
MH  - Water Pollutants/*poisoning
EDAT- 2003/09/10 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/09/10 05:00
PHST- 2003/09/10 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/09/10 05:00 [entrez]
AID - S0141-1136(03)00059-X [pii]
AID - 10.1016/S0141-1136(03)00059-X [doi]
PST - ppublish
SO  - Mar Environ Res. 2004 Feb-Mar;57(1-2):37-54. doi: 10.1016/S0141-1136(03)00059-X.