PMID- 12963965
OWN - NLM
STAT- MEDLINE
DCOM- 20040709
LR  - 20061115
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 23
IP  - 4
DP  - 2003 Oct
TI  - Genotypic analysis of esophageal squamous cell carcinoma by molecular 
      cytogenetics and real-time quantitative polymerase chain reaction.
PG  - 871-81
AB  - We performed an integrated cytogenetic study using a combination of comparative 
      genomic hybridization (CGH), spectral karyotyping (SKY) and fluorescence in situ 
      hybridization (FISH) to analyze chromosomal aberrations associated with 8 human 
      esophageal squamous cell carcinoma (EC-SCC) cell lines, and used real-time 
      quantitative PCR (Q-PCR) to study the copy number changes of two candidate genes 
      of chromosome 3q, PIK3CA and TP63, in 20 primary tumors of EC-SCC. The pooled CGH 
      results revealed frequent gain abnormalities on chromosome arms 1p, 1q, 3q, 5p, 
      6p, 7p, 7q, 8q, 9q, 11q, 12p, 14q, 15q, 16p, 16q, 17q, 18p, 19q, 20q, 22q, and 
      Xq, while frequent losses were found on 3p, 4, 5q, 6q, 7q, 9p, and 18q. SKY 
      detected 195 translocations, 13 deletions and 2 duplications. Among the 374 
      breakpoints, most clustered at the centromeric regions, such as 8q10, 13q10, 
      7q10, 9q10, 14q10, 15q10, 16q10, 21q10, and 22q10, but also at other regions, 
      including 3q (3q21, 3q22, 3q25), 7p (7p22, 7p14, 7p12), 7q (7q21, 7q31, 7q32), 8q 
      (8q21.1, 8q23), 11q (11q21, 11q24), 13q (13q14) and 18q (18q21). There was a good 
      correlation between the number of aberrations identified by CGH and SKY (r=0.667; 
      p=0.035). Combined CGH and SKY analyses indicated that chromosomes 3, 7, 9, 11, 
      14, 16, 18, 19, 20, and 22 harbored higher frequency of chromosomal aberrations 
      than expected. FISH using BAC clones containing oncogene PIK3CA and TP63 found 
      that both genes were amplified in 6 and 5 cell lines, respectively. Q-PCR 
      analysis of primary tumors revealed amplification of PIK3CA and TP63 in 100% and 
      80% of the cases. Average copy number of PIK3CA per haploid genome was greater 
      than that of TP63 (6.27 vs 2.73), and the difference showed statistical 
      significance (p<0.001). Combination of CGH, SKY and FISH could reveal detailed 
      chromosomal changes associated with esophageal cancer cells, and Q-PCR could 
      assess the change of the candidate genes in clinical samples in a high throughput 
      way.
FAU - Yen, Chueh-Chuan
AU  - Yen CC
AD  - Division of Medical Oncology, Department of Medicine, Taipei Veterans General 
      Hospital, Taipei, Taiwan. linch@ym.edu.tw
FAU - Chen, Yann-Jang
AU  - Chen YJ
FAU - Lu, Kai-Hsi
AU  - Lu KH
FAU - Hsia, Jiun-Yi
AU  - Hsia JY
FAU - Chen, Jung-Ta
AU  - Chen JT
FAU - Hu, Cheng-Po
AU  - Hu CP
FAU - Chen, Po-Min
AU  - Chen PM
FAU - Liu, Jin-Hwang
AU  - Liu JH
FAU - Chiou, Tzeon-Jye
AU  - Chiou TJ
FAU - Wang, Wei-Shu
AU  - Wang WS
FAU - Yang, Muh-Hwa
AU  - Yang MH
FAU - Chao, Ta-Chung
AU  - Chao TC
FAU - Lin, Chi-Hung
AU  - Lin CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor
MH  - Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Artificial, Bacterial
MH  - Esophageal Neoplasms/*genetics
MH  - *Genotype
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Metaphase
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/09/10 05:00
MHDA- 2004/07/10 05:00
CRDT- 2003/09/10 05:00
PHST- 2003/09/10 05:00 [pubmed]
PHST- 2004/07/10 05:00 [medline]
PHST- 2003/09/10 05:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2003 Oct;23(4):871-81.