PMID- 12967195
OWN - NLM
STAT- MEDLINE
DCOM- 20040506
LR  - 20190916
IS  - 0253-6269 (Print)
IS  - 0253-6269 (Linking)
VI  - 26
IP  - 8
DP  - 2003 Aug
TI  - Identification of new urinary metabolites of byakangelicin, a component of 
      Angelicae dahuricae Radix, in rats.
PG  - 606-11
AB  - Byakangelicin, 
      9-(2,3-dihydroxy-2-methylbutoxy)-4-methoxy-7H-furo[3,2-g][l]benzopyran-7-one 
      (BKG), a component of Angelicae dahuricae Radix, is considered to be an inhibitor 
      of aldose reductase for the treatment of diabetic cataract. An analytical method 
      for the isolation of BKG developed by high-performance liquid chromatography has 
      been reported. No literature on the metabolism of BKG, however, has been found. 
      With the purpose of identifying new metabolites of BKG, BKG (100 mg/kg) was 
      orally administered to Sprague-Dawley rats via a gavage. Using a metabolic cage, 
      urine was collected for 24 h, and the urine samples were extracted by 
      liquid-liquid extraction. For structural identification of new urinary 
      metabolites of BKG, various instrumental analyses were conducted by 
      gas-chromatography/mass spectrometry, high-performance liquid 
      chromatography/diode array detector, liquid chromatography/mass spectroscopy with 
      thermospray interface and 1H nuclear magnetic resonance spectroscopy. Two 
      metabolites produced from the O-demethylation or O-dealkylation of BKG were newly 
      identified, and another new but unknown metabolite was assumed to be the 
      hydroxylated form of BKG. These results indicate that the major metabolic 
      products of BKG are formed by O-demethylation or O-dealkylation of BKG side 
      chains.
FAU - Kwon, Oh-Seung
AU  - Kwon OS
AD  - Toxicology Lab., Bioanalysis and Biotransformation Research Center, Korea 
      Institute of Science and Technology, Seoul 136-791, Korea.
FAU - Song, Yun-Seon
AU  - Song YS
FAU - Shin, Kuk-Hyun
AU  - Shin KH
FAU - Ryu, Jae-Chun
AU  - Ryu JC
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Arch Pharm Res
JT  - Archives of pharmacal research
JID - 8000036
SB  - IM
MH  - Administration, Oral
MH  - Angelica/*chemistry
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Gas Chromatography-Mass Spectrometry
MH  - Magnetic Resonance Spectroscopy
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/09/12 05:00
MHDA- 2004/05/07 05:00
CRDT- 2003/09/12 05:00
PHST- 2003/09/12 05:00 [pubmed]
PHST- 2004/05/07 05:00 [medline]
PHST- 2003/09/12 05:00 [entrez]
AID - 10.1007/BF02976709 [doi]
PST - ppublish
SO  - Arch Pharm Res. 2003 Aug;26(8):606-11. doi: 10.1007/BF02976709.