PMID- 12967335
OWN - NLM
STAT- MEDLINE
DCOM- 20031204
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 178
IP  - 3
DP  - 2003 Sep
TI  - Propylthiouracil-induced hypothyroidism is associated with increased tolerance of 
      the isolated rat heart to ischaemia-reperfusion.
PG  - 427-35
AB  - The present study investigated the response of the hypothyroid heart to 
      ischaemia-reperfusion. Hypothyroidism was induced in Wistar rats by oral 
      administration of propylthiouracil (0.05%) for 3 weeks (HYPO rats), while normal 
      animals (NORM) served as controls. Isolated hearts from NORM and HYPO animals 
      were perfused in Langendorff mode and subjected to zero-flow global ischaemia 
      followed by reperfusion (I/R). Post-ischaemic recovery of left ventricular 
      developed pressure was expressed as % of the initial value (LVDP%). Basal 
      expression of protein kinase C epsilon (PKCepsilon) and PKCdelta and 
      phosphorylation of p46 and p54 c-jun NH(2)-terminal kinases (JNKs) in response to 
      I/R were assessed by Western blotting. LVDP% was found to be significantly higher 
      in HYPO hearts than in NORM. At baseline, PKCepsilon expression was 1.4-fold more 
      in HYPO than in NORM hearts, P<0.05, while PKCdelta was not changed. Furthermore, 
      basal phospho-p54 and -p46 JNK levels were 2.2- and 2.6-fold more in HYPO than in 
      NORM hearts, P<0.05. In response to I/R, in NORM hearts, phospho-p54 and -p46 JNK 
      levels were 5.5- and 6.0-fold more as compared with the baseline values, P<0.05, 
      while they were not significantly altered in HYPO hearts. HYPO hearts seem to 
      display a phenotype of cardioprotection against ischaemia-reperfusion and this is 
      associated with basal PKCepsilon overexpression and attenuated JNK activation 
      after I/R.
FAU - Pantos, C
AU  - Pantos C
AD  - Department of Pharmacology, University of Athens, 75 Mikras Asias Avenue, 11527 
      Goudi, Athens, Greece. cpantos@cc.uoa.gr
FAU - Malliopoulou, V
AU  - Malliopoulou V
FAU - Mourouzis, I
AU  - Mourouzis I
FAU - Sfakianoudis, K
AU  - Sfakianoudis K
FAU - Tzeis, S
AU  - Tzeis S
FAU - Doumba, P
AU  - Doumba P
FAU - Xinaris, C
AU  - Xinaris C
FAU - Cokkinos, A D
AU  - Cokkinos AD
FAU - Carageorgiou, H
AU  - Carageorgiou H
FAU - Varonos, D D
AU  - Varonos DD
FAU - Cokkinos, D V
AU  - Cokkinos DV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 721M9407IY (Propylthiouracil)
RN  - EC 2.7.1.- (Prkce protein, rat)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C-epsilon)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western/methods
MH  - Hypothyroidism/*complications/metabolism
MH  - *JNK Mitogen-Activated Protein Kinases
MH  - MAP Kinase Kinase 4
MH  - Male
MH  - Mitogen-Activated Protein Kinase Kinases/analysis/metabolism
MH  - Models, Animal
MH  - Myocardial Contraction
MH  - Myocardial Reperfusion Injury/*complications/metabolism
MH  - Myocardium/metabolism
MH  - Perfusion
MH  - Phosphorylation
MH  - Propylthiouracil
MH  - Protein Kinase C/metabolism
MH  - Protein Kinase C-epsilon
MH  - Rats
MH  - Rats, Wistar
MH  - Ventricular Pressure
EDAT- 2003/09/12 05:00
MHDA- 2003/12/05 05:00
CRDT- 2003/09/12 05:00
PHST- 2003/09/12 05:00 [pubmed]
PHST- 2003/12/05 05:00 [medline]
PHST- 2003/09/12 05:00 [entrez]
AID - 10.1677/joe.0.1780427 [doi]
PST - ppublish
SO  - J Endocrinol. 2003 Sep;178(3):427-35. doi: 10.1677/joe.0.1780427.