PMID- 12967679
OWN - NLM
STAT- MEDLINE
DCOM- 20031009
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 73
IP  - 20
DP  - 2003 Oct 3
TI  - Aged garlic extract attenuates gentamicin induced renal damage and oxidative 
      stress in rats.
PG  - 2543-56
AB  - Gentamicin (GM) is an antibiotic whose clinical use is limited by its 
      nephrotoxicity. Experimental evidences suggest a role of reactive oxygen species 
      in GM-induced nephrotoxicity. Therefore, we investigated if aged garlic extract 
      (AGE), an antioxidant, has a protective role in this experimental model. Four 
      groups of male Wistar rats were studied: 1) Control (CT), injected subcutaneously 
      (s.c.) and intraperitoneally (i.p.) with saline, 2) GM, treated s.c. with GM (70 
      mg/kg/12 hours/4 days), 3) AGE, treated i.p with AGE (1.2 mL/kg/12 hours/6 days), 
      and 4) GM + AGE treated with GM and AGE. The treatment with AGE started two days 
      before the first dose of GM (GM + AGE group) or saline (AGE group). Animals were 
      sacrificed on day 5, and blood, urine, and kidneys were obtained. Nephrotoxicity 
      was made evident by: 1) the increase in blood urea nitrogen and plasma 
      creatinine, 2) the decrease in plasma glutathione peroxidase (GPx) activity and 
      the urinary increase in N-acetyl-beta-D-glucosaminidase activity and total 
      protein, and 3) necrosis of proximal tubular cells. These alterations were 
      prevented or ameliorated by AGE treatment. Furthermore, AGE prevented the 
      GM-induced increase in the renal levels of oxidative stress markers: 
      nitrotyrosine and protein carbonyl groups and the decrease in manganese 
      superoxide dismutase (Mn-SOD), GPx, and glutathione reductase (GR) activities. 
      The protective effect of AGE was associated with the decrease in the oxidative 
      stress and the preservation of Mn-SOD, GPx, and GR activities in renal cortex. 
      These data suggest that AGE may be a useful agent for the prevention of 
      GM-nephrotoxicity.
FAU - Maldonado, Perla D
AU  - Maldonado PD
AD  - Department of Biology, Faculty of Chemistry, Universidad Nacional Autonoma de 
      Mexico, Ciudad Universitaria, 04510 D.F., Mexico, Mexico.
FAU - Barrera, Diana
AU  - Barrera D
FAU - Medina-Campos, Omar N
AU  - Medina-Campos ON
FAU - Hernandez-Pando, Rogelio
AU  - Hernandez-Pando R
FAU - Ibarra-Rubio, Maria E
AU  - Ibarra-Rubio ME
FAU - Pedraza-Chaverri, Jose
AU  - Pedraza-Chaverri J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Gentamicins)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Plant Extracts)
RN  - 11062-77-4 (Superoxides)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*antagonists & inhibitors/metabolism/*toxicity
MH  - Blotting, Western
MH  - Body Weight/drug effects
MH  - Catalase/metabolism
MH  - Free Radical Scavengers/*pharmacology
MH  - Garlic/*chemistry
MH  - Gentamicins/*antagonists & inhibitors/metabolism/*toxicity
MH  - Indicators and Reagents
MH  - Kidney/metabolism
MH  - Kidney Cortex/drug effects/enzymology
MH  - Kidney Diseases/*chemically induced/*pathology
MH  - Kidney Glomerulus/pathology
MH  - Kidney Tubules/pathology
MH  - Male
MH  - Oxidative Stress/*physiology
MH  - Plant Extracts/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Superoxides/metabolism
MH  - Urination/drug effects
EDAT- 2003/09/12 05:00
MHDA- 2003/10/10 05:00
CRDT- 2003/09/12 05:00
PHST- 2003/09/12 05:00 [pubmed]
PHST- 2003/10/10 05:00 [medline]
PHST- 2003/09/12 05:00 [entrez]
AID - S002432050300609X [pii]
AID - 10.1016/s0024-3205(03)00609-x [doi]
PST - ppublish
SO  - Life Sci. 2003 Oct 3;73(20):2543-56. doi: 10.1016/s0024-3205(03)00609-x.