PMID- 12968750
OWN - NLM
STAT- MEDLINE
DCOM- 20030923
LR  - 20190522
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 11
IP  - 5
DP  - 1999 Sep
TI  - Characterization of antibody response to porcine reproductive and respiratory 
      syndrome virus ORF5 product following infection and evaluation of its diagnostic 
      use in pigs.
PG  - 391-5
AB  - The sensitivity and specificity of recombinant open reading frame 5 products used 
      in the Western blotting assay for confirmation of porcine reproductive and 
      respiratory syndrome virus (PRRSV) serologic status were evaluated. The 
      recombinant antigen-based assays were specifically compared with a commercial 
      enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV 
      antibody-negative reference sera (n = 30), 2) naturally infected pig sera (n = 
      40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) 
      sera submitted to 3 state diagnostic laboratories (n = 200). The recombinant 
      antigen assay yielded an average increased sensitivity of 10% over the commercial 
      PRRSV ELISA. The negative controls (group 1 sera) showed no difference between 
      the 2 assays. This comparison confirmed that the recombinant antigen-specific 
      assay was more sensitive than the commercial ELISA and is well suited for routine 
      confirmation of the presence of PRRSV antibodies.
FAU - Kwang, J
AU  - Kwang J
AD  - USDA/ARS, Meat Animal Research Center, Clay Center, NE 68933, USA.
FAU - Yang, S
AU  - Yang S
FAU - Osorio, F A
AU  - Osorio FA
FAU - Christian, S
AU  - Christian S
FAU - Wheeler, J G
AU  - Wheeler JG
FAU - Lager, K M
AU  - Lager KM
FAU - Low, S
AU  - Low S
FAU - Chang, L
AU  - Chang L
FAU - Doster, A R
AU  - Doster AR
FAU - White, A
AU  - White A
FAU - Wu, C C
AU  - Wu CC
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Blotting, Western/*veterinary
MH  - Enzyme-Linked Immunosorbent Assay/*veterinary
MH  - Observer Variation
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/pathogenicity
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2003/09/13 05:00
MHDA- 2003/09/25 05:00
CRDT- 2003/09/13 05:00
PHST- 2003/09/13 05:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/09/13 05:00 [entrez]
AID - 10.1177/104063879901100501 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 1999 Sep;11(5):391-5. doi: 10.1177/104063879901100501.