PMID- 12969262
OWN - NLM
STAT- MEDLINE
DCOM- 20031112
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 87
IP  - 1
DP  - 2003 Oct
TI  - Hippocampal neurotrophin levels in a kainate model of temporal lobe epilepsy: a 
      lack of correlation between brain-derived neurotrophic factor content and 
      progression of aberrant dentate mossy fiber sprouting.
PG  - 147-59
AB  - A significant upregulation of neurotrophins particularly brain-derived 
      neurotrophic factor (BDNF) is believed to be involved in the initiation of 
      epileptogenic changes such as the aberrant axonal sprouting and synaptic 
      reorganization in the injured hippocampus. However, it is unknown which of the 
      neurotrophins are upregulated during the peak period of aberrant mossy fiber 
      sprouting in the chronically injured hippocampus. We measured chronic changes in 
      the levels of BDNF, nerve growth factor (NGF) and neurotrophin-3 (NT-3) in the 
      adult hippocampus using enzyme-linked immunosorbent assay (ELISA) after a 
      unilateral intracerebroventricular administration of kainic acid (KA), a model of 
      temporal lobe epilepsy. For comparison, neurotrophins were also measured from the 
      control intact hippocampus. Further, to see the association between changes in 
      neurotrophin levels and the progression of mossy fiber sprouting, chronic changes 
      in the mossy fiber distribution within the dentate supragranular layer (DSGL) 
      were quantified. In the KA-lesioned hippocampus, the neurotrophins BDNF and NGF 
      were upregulated at 4 days post-lesion, in comparison to their levels in the 
      intact hippocampus. However, the concentration of BDNF reached the baseline level 
      at 45 days post-lesion and dramatically diminished at 120 days post-lesion. In 
      contrast, the upregulation of NGF observed at 4 days post-lesion was sustained at 
      both 45 days and 120 days post-lesion. The concentration of NT-3 was upregulated 
      at 45 days post-lesion but remained comparable to baseline levels at 4 days and 
      120 days post-lesion. Interestingly, analysis of mossy fiber sprouting revealed 
      that most of the aberrant sprouting in the lesioned hippocampus occurs between 45 
      days and 120 days post-lesion. Taken together, these results suggest that the 
      period of robust mossy fiber sprouting does not correlate with the phase of 
      post-lesion BDNF upregulation. Rather, it shows a relationship with the time of 
      upregulation of neurotrophins NGF and NT-3.
FAU - Shetty, Ashok K
AU  - Shetty AK
AD  - Medical Research Service, Veterans Affairs Medical Center, Durham, North 
      Carolina, USA. Ashok.Shetty@duke.edu
FAU - Zaman, Vandana
AU  - Zaman V
FAU - Shetty, Geetha A
AU  - Shetty GA
LA  - eng
GR  - R01AG20924/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/drug effects
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Epilepsy, Temporal Lobe/chemically induced/*metabolism/pathology
MH  - Hippocampus/drug effects/*metabolism/pathology
MH  - Injections, Intraventricular
MH  - Kainic Acid
MH  - Male
MH  - Mossy Fibers, Hippocampal/drug effects/*pathology
MH  - Nerve Growth Factor/metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Neurotrophin 3/metabolism
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 2003/09/13 05:00
MHDA- 2003/11/13 05:00
CRDT- 2003/09/13 05:00
PHST- 2003/09/13 05:00 [pubmed]
PHST- 2003/11/13 05:00 [medline]
PHST- 2003/09/13 05:00 [entrez]
AID - 1979 [pii]
AID - 10.1046/j.1471-4159.2003.01979.x [doi]
PST - ppublish
SO  - J Neurochem. 2003 Oct;87(1):147-59. doi: 10.1046/j.1471-4159.2003.01979.x.