PMID- 12969525
OWN - NLM
STAT- MEDLINE
DCOM- 20040819
LR  - 20150313
VI  - 22
IP  - 9
DP  - 2003 Sep
TI  - [Suppressive effect of brain derived neurotrophic factor on H2O2-induced 
      apoptosis in human lung cancer cell YTMLC-90].
PG  - 938-42
AB  - BACKGROUND & OBJECTIVE: It has been shown that neurotrophic factors such as nerve 
      growth factor (NGF), brain derived neurotrophic factor (BDNF), and neurotrophin 
      (NT-3/4) are synthesized in a variety of cells inside and outside the nervous 
      system. These factors are not only able to promote neural survival, proliferation 
      and apoptosis of neural cells but also relevant to the activity of neural 
      invasion of tumors. It has not been reported to date whether BDNF may play roles 
      in the biological behavior of human lung cancer cells. The aim of this experiment 
      was to investigate the effect of BDNF on hydrogen peroxide (H2O2)-induced 
      apoptosis in the human lung cancer cell line YTMLC-90. METHODS: The minigene 
      pSVCEPBFCAT containing the promoter and enhancer elements of the human a1(I) 
      collagen gene(COLIA1) at its 3' terminus followed by hBDNF gene cDNA was 
      transfected and derived BDNF ectopic expression in the human lung cancer cells. 
      The cell proliferation was measured by MTT assay. The morphological and 
      ultra-structural changes of apoptotic cells were observed by microscopy with 
      fluorescent stain of acridine orange and electron microscopy. The DNA 
      fragmentation was examined by agarose gel electrophoresis. RESULTS: After 
      exposure of growing cells to 200 micromol/L H2O2 for 24 hours, the inhibition 
      rate of cell growth was 30% in the pSVCEPBFCAT-transfected YTMLC-90, 84.60% in 
      controls of non-transfected YTMLC-90, and 80.00% in pSVCEPCAT-transfected 
      YTMLC-90, respectively (P< 0.001). The morphological and biochemical changes of 
      apoptotic cells such as shrinkage of cytoplasm and nucleus,fragmentation of the 
      chromatin, and ladder pattern of DNA were commonly observed in the cell 
      population of controls, but these apoptotic features were not discovered in the 
      pSVCEPBFCAT-transfected YTMLC-90. CONCLUSION: BDNF markedly inhibits H2O2 
      cytotoxicity on human lung cancer cell YTMLC-90 by promoting YTMLC-90 
      proliferation and antagonizing H2O2-induced apoptosis.
FAU - Chen, Jian-Ye
AU  - Chen JY
AD  - Institute of Biochemistry and Molecular Biology, West China School of Preclinical 
      and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041, PR China.
FAU - Liu, Ji
AU  - Liu J
FAU - Wang, Ruo-Han
AU  - Wang RH
FAU - Peng, Wen-Zhen
AU  - Peng WZ
FAU - Liu, Zhi-Min
AU  - Liu ZM
FAU - Wang, Xiao-Ming
AU  - Wang XM
FAU - Chen, Hai-Yan
AU  - Chen HY
FAU - Chen, Jun-Jie
AU  - Chen JJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Ai Zheng
JT  - Ai zheng = Aizheng = Chinese journal of cancer
JID - 9424852
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Division/drug effects
MH  - Cell Line, Tumor
MH  - DNA Fragmentation
MH  - Humans
MH  - Hydrogen Peroxide/antagonists & inhibitors/*pharmacology
MH  - Lung Neoplasms/*pathology
EDAT- 2003/09/13 05:00
MHDA- 2004/08/20 05:00
CRDT- 2003/09/13 05:00
PHST- 2003/09/13 05:00 [pubmed]
PHST- 2004/08/20 05:00 [medline]
PHST- 2003/09/13 05:00 [entrez]
AID - 1000467X2003090938 [pii]
PST - ppublish
SO  - Ai Zheng. 2003 Sep;22(9):938-42.