PMID- 12969769
OWN - NLM
STAT- MEDLINE
DCOM- 20040803
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 476
IP  - 3
DP  - 2003 Aug 29
TI  - Nerve-mediated bladder contraction is impaired by cytokines: involvement of 
      inducible nitric oxide synthase.
PG  - 221-7
AB  - We investigated the possible involvement of inducible nitric oxide synthase 
      (iNOS) in the effect of cytokines on neuromuscular function in isolated rat 
      bladder strips. Bladder strips were incubated in cell culture medium for 24 h 
      with or without tumour necrosis factor-alpha (TNF-alpha)+interleukin-1beta. 
      Mechanical activity in response to electrical field stimulation and carbachol was 
      recorded in organ baths. Both the electrical field stimulation- and 
      carbachol-induced contractions were reduced by the incubation. The electrical 
      field stimulation-induced contraction was significantly further impaired after 
      prolonged exposure to TNF-alpha+interleukin-1beta. This impairment was restored 
      by dexamethasone, the iNOS inhibitor aminoguanidine and partially by 
      brain-derived neurotrophic factor (BDNF). In contrast, carbachol-induced 
      contractions were not affected by cytokines. iNOS protein expression was detected 
      in cytokine-incubated bladder strips by immunohistochemistry and Western blot 
      analysis. The results demonstrated that TNF-alpha+interleukin-1beta impaired 
      nerve-mediated bladder contractions. Aminoguanidine, and to some extent BDNF, 
      exerted neuroprotective effects.
FAU - Johansson, Rebecka
AU  - Johansson R
AD  - Department of Clinical Pharmacology, Lund University Hospital, SE-221 85 Lund, 
      Sweden.
FAU - Andersson, Karl-Erik
AU  - Andersson KE
FAU - Persson, Katarina
AU  - Persson K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cytokines/*metabolism/pharmacology
MH  - Electric Stimulation
MH  - Female
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Interleukin-1/metabolism/pharmacology
MH  - Muscle Contraction/physiology
MH  - Muscle, Smooth/enzymology/*physiology
MH  - Nitric Oxide Synthase/antagonists & inhibitors/*metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tumor Necrosis Factor-alpha/metabolism/pharmacology
MH  - Urinary Bladder/enzymology/*innervation/*physiology
EDAT- 2003/09/13 05:00
MHDA- 2004/08/04 05:00
CRDT- 2003/09/13 05:00
PHST- 2003/09/13 05:00 [pubmed]
PHST- 2004/08/04 05:00 [medline]
PHST- 2003/09/13 05:00 [entrez]
AID - S0014299903021782 [pii]
AID - 10.1016/s0014-2999(03)02178-2 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2003 Aug 29;476(3):221-7. doi: 10.1016/s0014-2999(03)02178-2.