PMID- 1297003 OWN - NLM STAT- MEDLINE DCOM- 19930429 LR - 20190918 IS - 0021-5112 (Print) IS - 0021-5112 (Linking) VI - 45 IP - 4 DP - 1992 Aug TI - Epidemiological study of malaria in north Sulawesi, Indonesia by fluorescence and Giemsa staining. PG - 175-84 AB - An epidemiological study of malaria infection was conducted in the Likupang District, Minahasa Regency, North Sulawesi Province, Indonesia, during August 2-15, 1991. In this study, 510 people of six villages, representing ages between 1 month to 84 years cooperated voluntarily. Blood smears stained with Giemsa and acridine orange (AO), revealed 33 and 83 malaria parasite positives respectively. This significant difference was due particularly to the fact that AO staining examined under either a daylight- or halogen-illuminated microscope equipped with interference filters was sensitive to detect low-density parasitemia in many subjects previously diagnosed negative by Giemsa staining in the field. The low malaria prevalence obtained by Giemsa staining may have been attributable to the lack of standard-quality diagnostic tools in the field or inadequate observation of the slides. In both staining methods, Plasmodium falciparum was found to be the predominant species, while the remainings were P. vivax or a mixture of both. Subjects infected with P. vivax revealed higher density of parasitemia and gametocytemia than those with P. falciparum. FAU - Syafruddin AU - Syafruddin AD - Laboratory of Parasitology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan. FAU - Kamimura, K AU - Kamimura K FAU - Hasegawa, H AU - Hasegawa H FAU - Toma, T AU - Toma T FAU - Miyagi, I AU - Miyagi I FAU - Kawamoto, F AU - Kawamoto F FAU - Nainggolan, I J AU - Nainggolan IJ FAU - Tumewu-Wagey, M AU - Tumewu-Wagey M FAU - Mandagi-Waworuntu, H AU - Mandagi-Waworuntu H FAU - Kapojos, F X AU - Kapojos FX AU - et al. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Japan TA - Jpn J Med Sci Biol JT - Japanese journal of medical science & biology JID - 0243706 RN - 0 (Azure Stains) RN - F30N4O6XVV (Acridine Orange) SB - IM MH - Acridine Orange MH - Adolescent MH - Adult MH - Age Factors MH - Aged MH - Aged, 80 and over MH - Azure Stains MH - Child MH - Child, Preschool MH - Humans MH - Indonesia/epidemiology MH - Infant MH - Malaria, Falciparum/blood/*epidemiology/parasitology MH - Malaria, Vivax/blood/*epidemiology/parasitology MH - Middle Aged EDAT- 1992/08/01 00:00 MHDA- 1992/08/01 00:01 CRDT- 1992/08/01 00:00 PHST- 1992/08/01 00:00 [pubmed] PHST- 1992/08/01 00:01 [medline] PHST- 1992/08/01 00:00 [entrez] AID - 10.7883/yoken1952.45.175 [doi] PST - ppublish SO - Jpn J Med Sci Biol. 1992 Aug;45(4):175-84. doi: 10.7883/yoken1952.45.175.