PMID- 12970166
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20141120
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 144
IP  - 12
DP  - 2003 Dec
TI  - Corticotropin-releasing hormone (CRH)-induced thyrotropin release is directly 
      mediated through CRH receptor type 2 on thyrotropes.
PG  - 5537-44
AB  - CRH is known as the main stimulator of ACTH release. In representatives of all 
      nonmammalian vertebrates, CRH has also been shown to induce TSH secretion, acting 
      directly at the level of the pituitary. We have investigated which cell types and 
      receptors are involved in CRH-induced TSH release in the chicken (Gallus gallus). 
      Because a lack of CRH type 1 receptors (CRH-R1) on the chicken thyrotropes has 
      been previously reported, two hypotheses were tested using in situ hybridization 
      and perifusion studies: 1) TSH secretion might be induced in a paracrine way 
      involving melanocortins from the corticotropes; and 2) thyrotropes might express 
      another type of CRH-R. For the latter, we have cloned a partial cDNA encoding the 
      chicken CRH-R2. Neither alpha-melanotropin (alpha-MSH) nor its powerful analog 
      Nle4,d-Phe7-MSH could mimic the in vitro TSH-releasing effect of ovine CRH. The 
      nonselective melanocortin receptor blocker SHU91199 did not influence CRH- or 
      TRH-induced TSH secretion. On the other hand, we have found that thyrotropes 
      express CRH-R2 mRNA. The involvement of this CRH receptor in the response of 
      thyrotropes to CRH was further confirmed by the fact that TSH release was 
      stimulated by human urocortin III, a CRH-R2-specific agonist, whereas the TSH 
      response to CRH was completely blocked by the CRH-R blocker astressin and the 
      CRH-R2-specific antagonist antisauvagine-30. We conclude that CRH-induced TSH 
      secretion is mediated by CRH-R2 expressed on thyrotropes.
FAU - De Groef, Bert
AU  - De Groef B
AD  - Laboratories of Comparative Endocrinology, Catholic University of Leuven, Leuven, 
      Belgium. bert.degroef@bio.kuleuven.ac.be.
FAU - Goris, Nesya
AU  - Goris N
FAU - Arckens, Lutgarde
AU  - Arckens L
FAU - Kuhn, Eduard R
AU  - Kuhn ER
FAU - Darras, Veerle M
AU  - Darras VM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030911
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (DNA, Complementary)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Corticotropin)
RN  - 0 (Urocortins)
RN  - 0 (antisauvagine 30)
RN  - 170809-51-5 (astressin)
RN  - 581-05-5 (alpha-MSH)
RN  - 9002-71-5 (Thyrotropin)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chickens
MH  - Cloning, Molecular
MH  - Corticotropin-Releasing Hormone/*metabolism/pharmacology
MH  - DNA, Complementary/genetics
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - Neuroprotective Agents/pharmacology
MH  - Peptide Fragments/pharmacology
MH  - Pituitary Gland/physiology
MH  - RNA, Messenger/analysis
MH  - Receptors, Corticotropin/antagonists & inhibitors/*genetics/*metabolism
MH  - Thyrotropin/*metabolism
MH  - Urocortins
MH  - alpha-MSH/pharmacology
EDAT- 2003/09/13 05:00
MHDA- 2004/01/06 05:00
CRDT- 2003/09/13 05:00
PHST- 2003/09/13 05:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/09/13 05:00 [entrez]
AID - en.2003-0526 [pii]
AID - 10.1210/en.2003-0526 [doi]
PST - ppublish
SO  - Endocrinology. 2003 Dec;144(12):5537-44. doi: 10.1210/en.2003-0526. Epub 2003 Sep 
      11.