PMID- 12971481
OWN - NLM
STAT- MEDLINE
DCOM- 20040211
LR  - 20061115
IS  - 0125-1562 (Print)
IS  - 0125-1562 (Linking)
VI  - 33 Suppl 3
DP  - 2002
TI  - Recent advances in basic and applied science for the control of 
      taeniasis/cysticercosis in Asia.
PG  - 79-82
AB  - Detection of seven specific bands by immunoblot (IB) using glycoproteins (GPs) 
      purified by lentil-lectin affinity chromatography has been the gold-standard for 
      neurocysticercosis (NCC) serodiagnosis since 1989. However, due to the presence 
      of contaminants, it was impossible to apply the GPs to ELISA. Our group at 
      Asahikawa Medical College (AMC) succeeded in purifying the GPs by preparative 
      isoelectric focusing; these higher quality GPs were suitable for ELISA. Based on 
      the results of both IB and ELISA testing, developed at AMC for a field survey in 
      Irian Jaya, it became evident that that area had pandemic NCC. We found many NCC 
      patients, pigs full of cysts, and one dog infected with two cysts: these findings 
      were based on serology. Recently, we conducted another survey to detect of the 
      worm carriers of T. solium. Three of the 38 local people were positive by 
      copro-antigen specific to Taenia species; these three patients expelled segments 
      of Taenia spp and these were confirmed as those of T. solium by mitochondrial DNA 
      analysis. When viable eggs of any taeniid species could be obtained, they can be 
      developed into metacestodes in NOD-scid mice; it then becomes possible to analyze 
      morphological dynamics, metacestode antigenicity, the efficacy of new 
      metacestocidal drugs, and mitochondrial DNA. Mitochondrial DNA analysis of the 
      specimens obtained in Irian Jaya was compared with that of other isolates 
      worldwide. T. solium is now divided into two genotypes: the Asian type, and the 
      Africa-American type. Some aspects of the pathological differences between the 
      Asian and Africa-American types and the antigenic components of these two types 
      are discussed.
FAU - Ito, A
AU  - Ito A
AD  - Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan. 
      akiraito@asahikawa-med.ac.jp
FAU - Wandra, T
AU  - Wandra T
FAU - Subahar, R
AU  - Subahar R
FAU - Hamid, A
AU  - Hamid A
FAU - Yamasaki, H
AU  - Yamasaki H
FAU - Sako, Y
AU  - Sako Y
FAU - Mamuti, W
AU  - Mamuti W
FAU - Okamoto, M
AU  - Okamoto M
FAU - Nakaya, K
AU  - Nakaya K
FAU - Nakao, M
AU  - Nakao M
FAU - Ishikawa, Y
AU  - Ishikawa Y
FAU - Suroso, T
AU  - Suroso T
FAU - Craig, P S
AU  - Craig PS
FAU - Margono, S S
AU  - Margono SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Thailand
TA  - Southeast Asian J Trop Med Public Health
JT  - The Southeast Asian journal of tropical medicine and public health
JID - 0266303
RN  - 0 (Antigens, Helminth)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/genetics
MH  - Asia/epidemiology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Mice
MH  - Mice, SCID
MH  - Polymorphism, Genetic
MH  - Serologic Tests
MH  - Swine
MH  - Taenia solium/immunology
MH  - Taeniasis/diagnosis/epidemiology/*prevention & control/veterinary
RF  - 27
EDAT- 2003/09/16 05:00
MHDA- 2004/02/12 05:00
CRDT- 2003/09/16 05:00
PHST- 2003/09/16 05:00 [pubmed]
PHST- 2004/02/12 05:00 [medline]
PHST- 2003/09/16 05:00 [entrez]
PST - ppublish
SO  - Southeast Asian J Trop Med Public Health. 2002;33 Suppl 3:79-82.