PMID- 12972077
OWN - NLM
STAT- MEDLINE
DCOM- 20031205
LR  - 20190922
IS  - 0892-0362 (Print)
IS  - 0892-0362 (Linking)
VI  - 25
IP  - 5
DP  - 2003 Sep-Oct
TI  - Prenatal nicotine exposure does not cause Purkinje cell loss in the developing 
      rat cerebellar vermis.
PG  - 633-7
AB  - Smoking by pregnant women poses a potential risk on the unborn child because 
      nicotine can easily be transported from the maternal to the fetal physiological 
      system. Our previous studies have shown that developing brains are sensitive to 
      nicotine-induced cell loss if nicotine was administered during the brain growth 
      spurt (early postnatal) in a rat model system. The purpose of this study was to 
      examine whether nicotine exposure prenatally (first two trimesters equivalent in 
      rats) would lead to Purkinje cell loss in the developing cerebellar vermis. 
      Pregnant female rats were subcutaneously implanted with 0 (placebo), 15 (NIC 15), 
      or 25 (NIC 25) mg nicotine pellets (21-day time released) on gestational day (GD) 
      0. An additional control group receiving no implantation was also introduced 
      (normal). One pup from each litter was sacrificed on postnatal day (PD) 10 and 
      the cerebellar vermis was processed for stereological cell counting of Purkinje 
      cells. No significant effects of prenatal nicotine treatment were found in the 
      forebrain, cerebellum, and brainstem weights. Similarly, the assessments of 
      volume, Purkinje cell number, and Purkinje cell density found no significant 
      differences among all treatment groups. Taken together, the current and a 
      previous finding, it suggests that there is a temporal window of vulnerability to 
      nicotine-induced Purkinje cell loss in the developing cerebellar vermis.
FAU - Chen, Wei-Jung A
AU  - Chen WJ
AD  - Department of Human Anatomy and Medical Neurobiology, College of Medicine, Texas 
      A&M University System Health Science Center, 1426 Reynolds Medical Building, 
      College Station, TX 77843-1114, USA. wjchen@tamu.edu
FAU - Edwards, Russell B
AU  - Edwards RB
LA  - eng
GR  - NS39899/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neurotoxicol Teratol
JT  - Neurotoxicology and teratology
JID - 8709538
RN  - 0 (Drug Implants)
RN  - 0 (Nicotinic Agonists)
RN  - 6M3C89ZY6R (Nicotine)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Body Weight
MH  - Brain/anatomy & histology
MH  - Cell Count
MH  - Cell Death/*drug effects
MH  - Cerebellum/cytology/*drug effects/embryology
MH  - Dose-Response Relationship, Drug
MH  - Drug Implants/administration & dosage
MH  - Female
MH  - Male
MH  - Nicotine/*toxicity
MH  - Nicotinic Agonists/*toxicity
MH  - Organ Size
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Purkinje Cells/*drug effects
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/09/16 05:00
MHDA- 2003/12/06 05:00
CRDT- 2003/09/16 05:00
PHST- 2003/09/16 05:00 [pubmed]
PHST- 2003/12/06 05:00 [medline]
PHST- 2003/09/16 05:00 [entrez]
AID - S0892036203000485 [pii]
AID - 10.1016/s0892-0362(03)00048-5 [doi]
PST - ppublish
SO  - Neurotoxicol Teratol. 2003 Sep-Oct;25(5):633-7. doi: 
      10.1016/s0892-0362(03)00048-5.