PMID- 12972510
OWN - NLM
STAT- MEDLINE
DCOM- 20040114
LR  - 20111117
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 74
IP  - 6
DP  - 2003 Dec
TI  - Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa 
      antigen of Mycobacterium tuberculosis.
PG  - 1008-14
AB  - Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are 
      potentially important vaccine target molecules. We used a motif-based strategy to 
      screen selected proteins of M. tuberculosis for peptides predicted to bind to 
      human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptides 
      that elicited cytolytic T lymphocyte activity and interferon-gamma production by 
      CD8+ T cells from HLA-A*0201+ healthy tuberculin reactors. These peptides were 
      derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a 
      novel target for CD8+ T cells. We speculate that hemolysins may alter the 
      phagosomal membrane surrounding intracellular M. tuberculosis, allowing 
      themselves and other antigens to gain access to the major histocompatibility 
      complex class I processing pathway.
FAU - Shams, Homayoun
AU  - Shams H
AD  - Center for Pulmonary and Infectious Disease Control, University of Texas Health 
      Center, 11937 U.S. Hwy. 271, Tyler, TX 75708-3154, USA. amir.shams@uthct.edu
FAU - Barnes, Peter F
AU  - Barnes PF
FAU - Weis, Stephen E
AU  - Weis SE
FAU - Klucar, Peter
AU  - Klucar P
FAU - Wizel, Benjamin
AU  - Wizel B
LA  - eng
GR  - A127285/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20030912
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Hemolysin Proteins)
RN  - 0 (Peptide Fragments)
RN  - 144058-44-6 (Mycobacterium tuberculosis antigens)
SB  - IM
MH  - Adult
MH  - Antigens, Bacterial/*immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - HLA-A Antigens/metabolism
MH  - HLA-A2 Antigen
MH  - Hemolysin Proteins/*immunology
MH  - Humans
MH  - Molecular Weight
MH  - Mycobacterium tuberculosis/*immunology
MH  - Peptide Fragments/metabolism
MH  - T-Lymphocytes, Cytotoxic/immunology
EDAT- 2003/09/16 05:00
MHDA- 2004/01/15 05:00
CRDT- 2003/09/16 05:00
PHST- 2003/09/16 05:00 [pubmed]
PHST- 2004/01/15 05:00 [medline]
PHST- 2003/09/16 05:00 [entrez]
AID - jlb.0403138 [pii]
AID - 10.1189/jlb.0403138 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2003 Dec;74(6):1008-14. doi: 10.1189/jlb.0403138. Epub 2003 Sep 
      12.