PMID- 12973805
OWN - NLM
STAT- MEDLINE
DCOM- 20041116
LR  - 20131121
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 24
IP  - 17
DP  - 2003 Sep
TI  - Microchip-based capillary electrochromatography using packed beds.
PG  - 3018-25
AB  - Integration of a packed column onto a microchip for performance of capillary 
      electrochromatography (CEC) is described. The quartz device incorporated a 
      cross-injector, and a double weir trapping design for formation of 1, 2 and 5 mm 
      long CEC columns. Three fluorescent dyes were baseline-resolved with plate 
      numbers of 330,(330,000 plates/m; height equivalent to a theoretical plate, H = 
      3.0 microm) for BODIPY 493/503, 360 (360,000 plates/m; H = 2.8 microm) for 
      rhodamine 123 and 244 (244,000 plates/m; H = 4.1 microm) for acridine orange (AO) 
      with 500 V applied on a 1 mm long column. The 2 mm column yielded approximately 
      1.8 times more theoretical plates than did the 1 mm column, when operated at the 
      same flow rate. Van Deemter plots were obtained for the three column lengths, 
      showing increased plate height for the 5 mm length. A 2 mm column gave peak 
      height and area relative standard deviation (RSD) values of 2.5 and 3.3%, 
      respectively, as averages for the three dyes (n = 15). The RSD for the dye 
      retention times was 1% (n = 6) over one day, and 3% (n = 30) over five days. 
      Indirect fluorescence detection of thiourea and of amino acids was possible using 
      a neutral indicator dye (BODIPY 493/503), with a detection limit of 10 microM for 
      amino acids.
FAU - Jemere, Abebaw B
AU  - Jemere AB
AD  - Department of Chemistry, University of Alberta, Edmonton, AB, Canada.
FAU - Oleschuk, Richard D
AU  - Oleschuk RD
FAU - Harrison, D Jed
AU  - Harrison DJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
RN  - 0 (Boron Compounds)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Methacrylates)
RN  - 0 (Polymers)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 77030-95-6 (octadecyl silane-bonded silica)
RN  - 94ZLA3W45F (Arginine)
RN  - GMW67QNF9C (Leucine)
RN  - GYV9AM2QAG (Thiourea)
SB  - IM
MH  - Arginine/analysis
MH  - Boron Compounds
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Electrophoresis, Capillary/instrumentation/*methods
MH  - Fluorescent Dyes
MH  - Lasers
MH  - Leucine/analysis
MH  - Methacrylates/chemistry
MH  - Microspheres
MH  - Polymers/chemistry
MH  - Reproducibility of Results
MH  - Silicon Dioxide
MH  - Thiourea/analysis
MH  - Time Factors
EDAT- 2003/09/16 05:00
MHDA- 2004/11/17 09:00
CRDT- 2003/09/16 05:00
PHST- 2003/09/16 05:00 [pubmed]
PHST- 2004/11/17 09:00 [medline]
PHST- 2003/09/16 05:00 [entrez]
AID - 10.1002/elps.200305538 [doi]
PST - ppublish
SO  - Electrophoresis. 2003 Sep;24(17):3018-25. doi: 10.1002/elps.200305538.