PMID- 12975779
OWN - NLM
STAT- MEDLINE
DCOM- 20040129
LR  - 20191210
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 23
IP  - 9
DP  - 2003 Sep
TI  - Fetal nucleated red blood cells in peripheral blood of pregnant women: detection 
      and determination of location on a slide using laser-scanning cytometry.
PG  - 710-5
AB  - OBJECTIVE: The purpose of the study was to assess the feasibility of analysis of 
      fetal nucleated red blood cells (NRBC) present in the maternal circulation by 
      laser-scanning cytometry. METHODS: CD71-positive cells were obtained by magnetic 
      cell sorting of peripheral blood of pregnant women after density centrifugation. 
      Immunofluorescence for the Hbgamma-chain was combined with fluorescent staining 
      of DNA (TO-PRO-3) and fluorescence in situ hybridization (FISH) with a 
      Y-chromosome specific probe. The cells were scanned on a slide using a 
      laser-scanning cytometer (LSC). Events double positive for Hbgamma and TO-PRO-3 
      were relocated and their morphology and FISH reactivity were visually assessed. 
      Determination of male fetal sex with LSC was compared with findings from 
      amniocentesis. RESULTS: In 8/15 pregnancies with male fetuses and in 0/9 with 
      females (apart from one case with a male/female twin pregnancy), we detected 
      Y-chromosome-positive NRBC. In pregnancies with female fetuses, 
      Y-chromosome-positive cells other than NRBC were found in all women who had 
      previously given birth to male babies, whereas women with no abortion and no male 
      babies in their history did not present with Y-chromosome-positive non-NRBC. 
      CONCLUSION: On the basis of automatic relocation of once-defined cells of fetal 
      origin from the current pregnancy, laser-scanning cytometry is likely to 
      facilitate repeated (poly-)FISH analysis and single-cell PCR for noninvasive 
      prenatal diagnosis.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Hennerbichler, Simone
AU  - Hennerbichler S
AD  - Institute of Histology and Embryology, Karl-Franzens-University Graz, Austria.
FAU - Kroisel, Peter M
AU  - Kroisel PM
FAU - Zierler, Hannelore
AU  - Zierler H
FAU - Pertl, Barbara
AU  - Pertl B
FAU - Wintersteiger, Reinhold
AU  - Wintersteiger R
FAU - Dohr, Gottfried
AU  - Dohr G
FAU - Sedlmayr, Peter
AU  - Sedlmayr P
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Y/genetics
MH  - *Erythroblasts
MH  - Female
MH  - Humans
MH  - Image Cytometry/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Maternal Age
MH  - Predictive Value of Tests
MH  - Pregnancy/*blood
MH  - Pregnancy Trimester, First/blood
MH  - Pregnancy Trimester, Second/blood
MH  - Pregnancy, High-Risk
MH  - *Prenatal Diagnosis
EDAT- 2003/09/17 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/09/17 05:00
PHST- 2003/09/17 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/09/17 05:00 [entrez]
AID - 10.1002/pd.668 [doi]
PST - ppublish
SO  - Prenat Diagn. 2003 Sep;23(9):710-5. doi: 10.1002/pd.668.