PMID- 1301164
OWN - NLM
STAT- MEDLINE
DCOM- 19930603
LR  - 20220409
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 1
IP  - 8
DP  - 1992 Nov
TI  - The organization of the intron-containing human S6 ribosomal protein (rpS6) gene 
      and determination of its location at chromosome 9p21.
PG  - 565-70
AB  - The intron-containing gene encoding human ribosomal protein S6 (rpS6), the major 
      phosphoprotein in the mammalian ribosome, has been cloned. Using a PCR based 
      cloning strategy we have isolated the rpS6 intron-containing gene in the presence 
      of its many processed pseudogenes and determined the DNA sequence of the entire 
      gene and its upstream and downstream flanking regions. The human rpS6 gene is 
      3979 bp in length and comprises six exons. Despite lacking a consensus TATA box, 
      primer extension analysis indicates that the start of transcription is located at 
      a single C residue within an 11 bp oligopyrimidine tract. The first exon, which 
      contains the ATG start codon, is 48 bp in length. The DNA sequence in the 5' 
      region of the gene has features of a CpG-rich island. Using fluorescence in situ 
      hybridization (FISH) analysis the position of the rpS6 gene has been sublocalized 
      to human chromosome 9p21. The similarities and differences between rpS6 and other 
      previously characterized ribosomal protein genes are discussed.
FAU - Antoine, M
AU  - Antoine M
AD  - Department of Eukaryotic Gene Organization and Expression, Imperial Cancer 
      Research Fund, London, UK.
FAU - Fried, M
AU  - Fried M
LA  - eng
SI  - GENBANK/M81930
SI  - GENBANK/M88632
SI  - GENBANK/M88633
SI  - GENBANK/M88634
SI  - GENBANK/M88635
SI  - GENBANK/M88636
SI  - GENBANK/M88637
SI  - GENBANK/X64598
SI  - GENBANK/X64599
SI  - GENBANK/X67309
PT  - Journal Article
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Ribosomal Protein S6)
RN  - 0 (Ribosomal Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - rpS6
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 9
MH  - DNA
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Introns
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Ribosomal Protein S6
MH  - Ribosomal Proteins/*genetics
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
AID - 10.1093/hmg/1.8.565 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1992 Nov;1(8):565-70. doi: 10.1093/hmg/1.8.565.