PMID- 1301582
OWN - NLM
STAT- MEDLINE
DCOM- 19930603
LR  - 20041117
IS  - 0361-7742 (Print)
IS  - 0361-7742 (Linking)
VI  - 378
DP  - 1992
TI  - Flow cytometric analysis and motility response to laminin and fibronectin of four 
      new metastatic variants of the human renal cell carcinoma line RC43.
PG  - 195-205
AB  - Using subrenal capsule implantation (SRC) in nude mice we have isolated 4 new 
      sublines (RC43MSP, MBO, MLI, MDI) from the human renal cell carcinoma line (HRCC) 
      RC43. These sublines exhibit an enhanced metastatic ability and a faster doubling 
      time after subcutaneous (SC) transplantation in vivo, when compared to the 
      parental line. With the aim of a better characterization of these new sublines we 
      have performed a flow cytometric analysis (FA) of the DNA content and studied the 
      motility response (MR) to laminin and fibronectin. FA results. The RC43 parental 
      line showed a tetraploid pattern (DNA index: 2.08). Also the variant lines 
      RC43MLI and RC43MDI were tetraploid (DNAind: 2.05 and 2.07, respectively). With 
      RC43MDI a second peak in the tetraploid range was evident. The RC43MSP and the 
      RC43MBO sublines both showed a hypertetraploid pattern (DNAind respectively 2.21 
      and 2.11). MR results. Motility studies showed that all RC43 lines, including the 
      parental line, were stimulated to migrate in response to either laminin or 
      fibronectin both from rat and human sources. There were no significant 
      differences between the parental line and its variants in this respect, however. 
      We conclude that by the use of FA at least two of the sublines (MSP and MBO) can 
      be distinguished from the parental RC43 cell line, suggesting a different clonal 
      origin of the various sublines. As all sublines, as well as the parental line, 
      showed a large but similar response to migration-stimulating agents (such as 
      laminin and fibronectin) determination of MR could not be used to obtain a 
      further discrimination between the different sublines.
FAU - Grossi, F S
AU  - Grossi FS
AD  - Department of Urology, Erasmus University Rotterdam, The Netherlands.
FAU - Keizer, D M
AU  - Keizer DM
FAU - Saracino, G A
AU  - Saracino GA
FAU - Erkens, S
AU  - Erkens S
FAU - Romijn, J C
AU  - Romijn JC
FAU - Schroder, F H
AU  - Schroder FH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Prog Clin Biol Res
JT  - Progress in clinical and biological research
JID - 7605701
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Fibronectins)
RN  - 0 (Laminin)
SB  - IM
MH  - Animals
MH  - Carcinoma, Renal Cell/genetics/pathology/*secondary
MH  - Cell Movement/drug effects
MH  - DNA, Neoplasm/genetics
MH  - Fibronectins/pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - *Kidney Neoplasms/genetics/pathology
MH  - Laminin/pharmacology
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Polyploidy
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured/drug effects/pathology
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PST - ppublish
SO  - Prog Clin Biol Res. 1992;378:195-205.