PMID- 1304461
OWN - NLM
STAT- MEDLINE
DCOM- 19930707
LR  - 20051117
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 105
IP  - 11
DP  - 1992 Nov
TI  - Effect of hamster liver passage on the isoenzyme patterns of Entamoeba 
      histolytica.
PG  - 918-22
AB  - The effect of hamster liver passage on the isoenzyme patterns of isolates of 
      Entamoeba histolytica was investigated. Three isolates, F, G and T were 
      originally obtained from patients with acute amebic dysentery and another strain, 
      C, was obtained from an asymptomatic carrier. All these strains were maintained 
      for over two years in axenic culture. The isoenzyme pattern (zymodeme) of 
      hexokinase (HK), phosphoglucomutase (PGM) and glucose phosphoisomerase (GPI) of 
      these strains was found to belong to non-pathogenic group X, but the isoenzyme 
      pattern of GPI resembled less pathogenic zymodeme XX and might be an intermediate 
      type. Following inoculation of trophozoites into hamster livers and recovery 
      after abscess formation, their isoenzyme pattern changed and revealed that they 
      belonged to pathogenic type XIV. Liver passage caused an enhancement in amebic 
      virulence as evidenced by their increased ability to destroy leukocytes. The 
      results indicate that isoenzyme pattern is not a stable property of E. 
      histolytica.
FAU - Cheng, X J
AU  - Cheng XJ
AD  - Department of Parasitology, Shanghai Medical University.
FAU - Huang, M Y
AU  - Huang MY
FAU - Mirelman, D
AU  - Mirelman D
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Isoenzymes)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
RN  - EC 5.4.2.2 (Phosphoglucomutase)
SB  - IM
MH  - Animals
MH  - Cricetinae
MH  - Entamoeba histolytica/*enzymology/pathogenicity
MH  - Glucose-6-Phosphate Isomerase/*metabolism
MH  - Hexokinase/metabolism
MH  - Isoenzymes/*metabolism
MH  - Liver/parasitology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phosphoglucomutase/metabolism
MH  - Serial Passage
MH  - Virulence
EDAT- 1992/11/01 00:00
MHDA- 1992/11/01 00:01
CRDT- 1992/11/01 00:00
PHST- 1992/11/01 00:00 [pubmed]
PHST- 1992/11/01 00:01 [medline]
PHST- 1992/11/01 00:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 1992 Nov;105(11):918-22.