PMID- 1307254
OWN - NLM
STAT- MEDLINE
DCOM- 19930831
LR  - 20190509
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 1
IP  - 7
DP  - 1992 Oct
TI  - Oligonucleotide probes for the analysis of specific repetitive DNA sequences by 
      fluorescence in situ hybridization.
PG  - 535-9
AB  - Five non-isotopically labeled oligonucleotides have been designed and synthesized 
      to facilitate the analysis of specific human and murine repetitive DNA sequences 
      by fluorescence in situ hybridization (FISH). Three of the oligonucleotides 
      contain alphoid DNA sequences; one hybridizes to the centromeres of all human and 
      mouse chromosomes except the Y, while the other two are specific for human 
      chromosomes 2 and 12, respectively. The fourth oligomer, containing sequences 
      from the spacer region of a human 5S rDNA repeat, was used to confirm the map 
      location of a approximately 100 copy 5S rDNA tandem repeat locus. The fifth 
      oligomer, specific to the Alu family of repeats, generates a sharp R-banding 
      pattern on human metaphase chromosomes, suitable for FISH karyotyping. These 
      probes permit highly specific chromosome enumeration and aneuploidy detection 
      with hybridization times as short as 30 minutes.
FAU - Matera, A G
AU  - Matera AG
AD  - Department of Genetics, Yale University School of Medicine, New Haven, CT 06510.
FAU - Ward, D C
AU  - Ward DC
LA  - eng
GR  - GM-40115/GM/NIGMS NIH HHS/United States
GR  - HG-00246/HG/NHGRI NIH HHS/United States
GR  - HG-00272/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Oligonucleotide Probes
MH  - RNA, Ribosomal, 5S/genetics
MH  - *Repetitive Sequences, Nucleic Acid
EDAT- 1992/10/01 00:00
MHDA- 1992/10/01 00:01
CRDT- 1992/10/01 00:00
PHST- 1992/10/01 00:00 [pubmed]
PHST- 1992/10/01 00:01 [medline]
PHST- 1992/10/01 00:00 [entrez]
AID - 10.1093/hmg/1.7.535 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1992 Oct;1(7):535-9. doi: 10.1093/hmg/1.7.535.