PMID- 13075
OWN - NLM
STAT- MEDLINE
DCOM- 19770315
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 252
IP  - 2
DP  - 1977 Jan 25
TI  - Assay and partial characterization of the solubilized cell surface receptor for 
      immunoglobulin E.
PG  - 704-11
AB  - The cell surface component (receptor) which specifically binds immunoglobulin E 
      (IgE) presumably forms an integral part of the functional chain involved in the 
      antigen-induced IgE-mediated degranulation of histamine-containing mast cells and 
      basophils. This paper describes a simple (NH4)2SO4 predipitation assay with which 
      the interaction of IgE with detergent-solubilized receptors can be reproducibly 
      quantitated. Receptor saturation was demonstrated and a linear response to 
      receptor concentration over at least a 30-fold rang obtained. By means of the 
      assay it was shown that (a) all assayable receptors of rat basophil leukemia 
      cells are cell surface expressed; (b) receptor specificity remains intact during 
      solubilization; (c) the binding constants of the solubilized IgE receptors are 
      similar to those determined on intact cells. Utilizing agarose gel filtration, 
      preliminary estimates of the molecular weight of the active free solubilized 
      receptor and of its complex with IgE suggest that the receptor is univalent.
FAU - Rossi, G
AU  - Rossi G
FAU - Newman, S A
AU  - Newman SA
FAU - Metzger, H
AU  - Metzger H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Detergents)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - SU46BAM238 (Ammonium Sulfate)
SB  - IM
MH  - Ammonium Sulfate/pharmacology
MH  - Binding Sites, Antibody
MH  - Cell Membrane/*immunology
MH  - Detergents/pharmacology
MH  - Dose-Response Relationship, Immunologic
MH  - Hydrogen-Ion Concentration
MH  - Immunoassay
MH  - *Immunoglobulin E/metabolism
MH  - Kinetics
MH  - Mast Cells/*immunology
MH  - Osmolar Concentration
MH  - Polyethylene Glycols/pharmacology
EDAT- 1977/01/25 00:00
MHDA- 1977/01/25 00:01
CRDT- 1977/01/25 00:00
PHST- 1977/01/25 00:00 [pubmed]
PHST- 1977/01/25 00:01 [medline]
PHST- 1977/01/25 00:00 [entrez]
AID - S0021-9258(17)32775-8 [pii]
PST - ppublish
SO  - J Biol Chem. 1977 Jan 25;252(2):704-11.