PMID- 1309726
OWN - NLM
STAT- MEDLINE
DCOM- 19920220
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 50
IP  - 2
DP  - 1992 Jan 21
TI  - Local growth of a Burkitt's lymphoma versus disseminated invasive growth of the 
      autologous EBV-immortalized lymphoblastoid cells and their somatic cell hybrids 
      in SCID mice.
PG  - 265-73
AB  - Specific host-graft interactions, as well as intrinsic properties of transferred 
      cell, determine tumorigenicity in xenogeneic systems. We compared the growth 
      characteristics of human B-lymphoid cell lines in SCID mice with the well 
      characterized growth pattern in nude mice and observed striking differences in 
      malignancy in the respective hosts. Two cell lines derived from the same 
      individual, the Epstein-Barr-virus(EBV)-positive Burkitt's lymphoma BL 60 (BL) 
      and the autologous EBV-immortalized lymphoblastoid cell line IARC 277 (LCL) were 
      used. In addition, we tested somatic cell hybrids (HYB) of both cell lines, which 
      despite the LCL-like differentiation phenotype show the de-regulated c-myc 
      expression pattern of the parental BL line, assumed to be a critical factor in BL 
      pathogenesis. Subcutaneously (s.c.) injected BL cells produced local 
      progressively growing tumor masses at the injection site without distant 
      metastases in both nude and SCID mice. Although both mouse strains possess the 
      same genetic background (BALB/c) and differ only in the B-cell sub-set, the 
      growth patterns of the LCL and hybrids were completely different. In contrast to 
      the regressive behaviour of LCL and hybrids in nude mice, these lines show 
      invasive and disseminated progressive growth in SCID mice. Peripheral lymph nodes 
      an thymic tissue were preferentially colonized, whereas mucosal-associated 
      lymphoid tissue (Peyer's patches and appendix) and spleen were not infiltrated. 
      The preferential migration of lymphocytes to certain tissues is termed homing in 
      a syngeneic system and mediated by homing receptors and vascular addressins. The 
      "homing" of LCL and hybrids into lymphoid SCID mouse tissue suggests a strong 
      interaction with the endothelial cells of the host. Detailed phenotypic analysis 
      of BL, LCL and 3 different hybrids was performed using an antibody panel against 
      differentiation and adhesion markers. Overall dominance of the LCL phenotype was 
      observed in the hybrids, as indicated by cytology, tumor growth, dissemination 
      and the pattern of surface-marker expression. The c-myc activation in hybrids 
      does not appear to influence growth behavior.
FAU - Walter, J
AU  - Walter J
AD  - Institute of Immunology and Genetics, German Cancer Research Center, Heidelberg.
FAU - Moller, P
AU  - Moller P
FAU - Moldenhauer, G
AU  - Moldenhauer G
FAU - Schirrmacher, V
AU  - Schirrmacher V
FAU - Pawlita, M
AU  - Pawlita M
FAU - Wolf, J
AU  - Wolf J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Immunoglobulins)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/analysis
MH  - B-Lymphocytes/pathology
MH  - Burkitt Lymphoma/immunology/*pathology
MH  - Cell Adhesion Molecules/analysis
MH  - Cell Division/physiology
MH  - *Cell Transformation, Viral
MH  - Child, Preschool
MH  - Female
MH  - Graft vs Host Reaction/physiology
MH  - *Herpesvirus 4, Human
MH  - Humans
MH  - Hybrid Cells
MH  - Immunoglobulins/analysis
MH  - Immunophenotyping
MH  - Lymphoproliferative Disorders/*pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Mice, SCID
MH  - Neoplasm Invasiveness/pathology
MH  - Neoplasm Metastasis/pathology
MH  - Neoplasm Transplantation
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
EDAT- 1992/01/21 00:00
MHDA- 1992/01/21 00:01
CRDT- 1992/01/21 00:00
PHST- 1992/01/21 00:00 [pubmed]
PHST- 1992/01/21 00:01 [medline]
PHST- 1992/01/21 00:00 [entrez]
AID - 10.1002/ijc.2910500217 [doi]
PST - ppublish
SO  - Int J Cancer. 1992 Jan 21;50(2):265-73. doi: 10.1002/ijc.2910500217.