PMID- 1310332
OWN - NLM
STAT- MEDLINE
DCOM- 19920302
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 30
IP  - 1
DP  - 1992 Jan
TI  - Susceptibility testing of clinical isolates of Enterococcus faecium and 
      Enterococcus faecalis.
PG  - 41-5
AB  - We collected 103 clinical Enterococcus faecium isolates from across Canada, 
      performed standard broth microdilution susceptibility testing, and compared these 
      results with results from the MicroScan Pos MIC Type 6 panel (Baxter Health Care 
      Corp., West Sacramento, Calif.) and the AMS-Vitek Gram-Positive Susceptibility 
      card (Vitek Inc., St. Louis, Mo.). High-level aminoglycoside resistance to 
      gentamicin and streptomycin was detected by a single-concentration agar method 
      with 1,000 micrograms of each aminoglycoside per ml. In addition, we tested the 
      effect of the lower calcium content in broth media as recommended in National 
      Committee for Clinical Laboratory Standards (NCCLS) guideline M7-A2 on the 
      activity of the highly calcium-dependent agent daptomycin. Of the 103 E. faecium 
      isolates, there were 4 and 30 isolates with high-level gentamicin resistance 
      (HLGR) and high-level streptomycin resistance (HLSR), respectively. An additional 
      39 (37 with HLGR and 36 with HLSR) E. faecium isolates were tested by both the 
      MicroScan and the AMS-Vitek systems. The AMS-Vitek card demonstrated 
      sensitivities of 95 and 82% for detecting HLGR strains and HLSR strains, 
      respectively. The MicroScan panel demonstrated improved sensitivities for 
      detecting HLGR (42 to 97%) and HLSR (64 to 84%) when readings were performed 
      manually instead of being generated automatically. Ampicillin resistance (MIC, 
      greater than or equal to 16 micrograms/ml) was detected in 23 of the 103 E. 
      faecium isolates. Only 14 and 20 of these were detected by the MicroScan panels 
      and AMS-Vitek cards, respectively. beta-Lactamase activity was not detected in 
      any isolates. The lower calcium content in broth media recommended by NCCLS 
      guideline M7-A2 markedly reduced the in vitro activity of daptomycin against 
      Enterococcus spp.
FAU - Louie, M
AU  - Louie M
AD  - Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada.
FAU - Simor, A E
AU  - Simor AE
FAU - Szeto, S
AU  - Szeto S
FAU - Patel, M
AU  - Patel M
FAU - Kreiswirth, B
AU  - Kreiswirth B
FAU - Low, D E
AU  - Low DE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Peptides)
RN  - NWQ5N31VKK (Daptomycin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium
MH  - Daptomycin
MH  - Drug Resistance, Microbial
MH  - Enterococcus faecalis/*drug effects/growth & development/isolation & purification
MH  - Enterococcus faecium/*drug effects/growth & development/isolation & purification
MH  - Humans
MH  - *Microbial Sensitivity Tests/standards
MH  - Peptides/pharmacology
PMC - PMC264993
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
PMCR- 1992/01/01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PHST- 1992/01/01 00:00 [pmc-release]
AID - 10.1128/jcm.30.1.41-45.1992 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1992 Jan;30(1):41-5. doi: 10.1128/jcm.30.1.41-45.1992.