PMID- 1311740
OWN - NLM
STAT- MEDLINE
DCOM- 19920407
LR  - 20131121
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 119
IP  - 3
DP  - 1992 Mar
TI  - Characterization of the ferritin receptors of human T lymphoid (MOLT-4) cells.
PG  - 273-9
AB  - We have previously demonstrated that distinct binding sites exist for human 
      recombinant H ferritin (HrHF) and human liver ferritin (HLF) on human T lymphoid 
      cells (MOLT-4). This study demonstrates that these binding sites have the 
      characteristics of receptors specific for HrHF, and the binding characteristics 
      and internalization of HrHF to MOLT-4 cells have now been examined. Iodinated 
      HrHF was displaced by an excess of unlabeled HrHF. Heavy ferritin was the major 
      subunit bound with only a small amount of light-ferritin binding, consistent with 
      our immunofluorescence studies. Scatchard plot analysis of the competitive 
      binding data for HrHF revealed an association constant of 6.3 to 6.7 x 10(7) 
      L/mol with approximately 6000 to 15,000 receptor sites per MOLT-4 cell. 
      Internalization of HrHF was demonstrated with pronase. Chloroquine substantially 
      reduced the uptake of HrHF. Release of internalized HrHF was not observed when 
      cells were rewarmed to 37 degrees C. These results indicate that HrHF is 
      internalized by a mechanism consistent with receptor-mediated endocytosis, with 
      possible involvement of the lysosome. The internalized HrHF remains associated 
      with the cell. Although lymphoid cell growth and differentiation were not 
      examined in this study, the presence of the demonstrated receptors may indicate a 
      regulatory role for heavy ferritin in such cells.
FAU - Moss, D
AU  - Moss D
AD  - Liver Unit, Queensland Institute of Medical Research, Brisbane, Australia.
FAU - Powell, L W
AU  - Powell LW
FAU - Arosio, P
AU  - Arosio P
FAU - Halliday, J W
AU  - Halliday JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (Iron-Binding Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (ferritin receptor)
RN  - 886U3H6UFF (Chloroquine)
RN  - 9007-73-2 (Ferritins)
SB  - IM
MH  - Binding, Competitive
MH  - Cells, Cultured
MH  - Chloroquine/pharmacology
MH  - Ferritins/metabolism
MH  - Humans
MH  - Iodine Radioisotopes
MH  - *Iron-Binding Proteins
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/pathology
MH  - Radioligand Assay
MH  - Receptors, Cell Surface/*analysis/metabolism/physiology
MH  - T-Lymphocytes/*chemistry/metabolism/*ultrastructure
MH  - Temperature
MH  - Time Factors
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
PST - ppublish
SO  - J Lab Clin Med. 1992 Mar;119(3):273-9.