PMID- 13129650
OWN - NLM
STAT- MEDLINE
DCOM- 20031216
LR  - 20181130
IS  - 0928-8244 (Print)
IS  - 0928-8244 (Linking)
VI  - 38
IP  - 2
DP  - 2003 Sep 22
TI  - Increased production of matrix metalloproteinases 1 and 3 by smooth muscle cells 
      upon infection with Chlamydia pneumoniae.
PG  - 159-64
AB  - Atherosclerosis has been linked to Chlamydia pneumoniae infection. In 
      atherosclerotic arteries chlamydiae infect macrophages, endothelial cells, and 
      smooth muscle cells (SMC). It has been suggested that the proteolysis of the 
      extracellular matrix by matrix metalloproteinases (MMPs) is involved in the 
      destabilisation and rupture of atherosclerotic plaques. In this study we 
      investigated the expression of several MMPs and tissue inhibitors of MMP (TIMPs) 
      in C. pneumoniae-infected SMC using reverse transcription-polymerase chain 
      reaction analysis. Chlamydial infection of SMC up-regulated the mRNA levels of 
      MMP-1 (interstitial collagenase) and MMP-3 (stromelysin) but did not affect the 
      expression of MMP-2 and -9 (gelatinases). Additionally, the levels of TIMP-1 and 
      -2 mRNA remained unchanged upon infection. Cells infected with C. pneumoniae 
      secreted increased quantities of MMP-1 and -3 proteins as demonstrated by 
      enzyme-linked immunosorbent assays. The ability of C. pneumoniae to stimulate the 
      production of MMP-1 and -3 by SMC may be important for its pathogenic role in the 
      progression of atherosclerotic disease.
FAU - Rodel, Jurgen
AU  - Rodel J
AD  - Institute of Medical Microbiology, Friedrich Schiller University of Jena, 
      Semmelweisstr. 4, D-07740 Jena, Germany. juergen.roedel@med.uni-jena.de
FAU - Prochnau, Dirk
AU  - Prochnau D
FAU - Prager, Katrin
AU  - Prager K
FAU - Pentcheva, Evdokia
AU  - Pentcheva E
FAU - Hartmann, Matthias
AU  - Hartmann M
FAU - Straube, Eberhard
AU  - Straube E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.- (Metalloproteases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Arteriosclerosis/microbiology/physiopathology
MH  - Cells, Cultured
MH  - Chlamydophila pneumoniae/*pathogenicity
MH  - Humans
MH  - Matrix Metalloproteinase 1/*biosynthesis/genetics
MH  - Matrix Metalloproteinase 3/*biosynthesis/genetics
MH  - Metalloproteases/metabolism
MH  - Muscle, Smooth, Vascular/cytology/*enzymology/microbiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinases/metabolism
MH  - *Up-Regulation
EDAT- 2003/09/18 05:00
MHDA- 2003/12/17 05:00
CRDT- 2003/09/18 05:00
PHST- 2003/09/18 05:00 [pubmed]
PHST- 2003/12/17 05:00 [medline]
PHST- 2003/09/18 05:00 [entrez]
AID - S0928824403001263 [pii]
AID - 10.1016/S0928-8244(03)00126-3 [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2003 Sep 22;38(2):159-64. doi: 
      10.1016/S0928-8244(03)00126-3.