PMID- 13129912
OWN - NLM
STAT- MEDLINE
DCOM- 20040203
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 278
IP  - 47
DP  - 2003 Nov 21
TI  - Adiposity elevates plasma MCP-1 levels leading to the increased CD11b-positive 
      monocytes in mice.
PG  - 46654-60
AB  - Obesity is currently considered as an epidemic in the western world, and it 
      represents a major risk factor for life-threatening diseases such as heart 
      attack, stroke, diabetes, and cancer. Taking advantage of DNA microarray 
      technology, we tried to identify the molecules explaining the relationship 
      between obesity and vascular disorders, comparing mRNA expression of about 12,000 
      genes in white adipose tissue between normal, high fat diet-induced obesity (DIO) 
      and d-Trp34 neuropeptide Y-induced obesity in mice. Expression of monocyte 
      chemoattractant protein-1 (MCP-1) mRNA displayed a 7.2-fold increase in obese 
      mice as compared with normal mice, leading to substantially elevated MCP-1 
      protein levels in adipocytes. MCP-1 levels in plasma were also increased in DIO 
      mice, and a strong correlation between plasma MCP-1 levels and body weight was 
      identified. We also showed that elevated MCP-1 protein levels in plasma increased 
      the CD11b-positive monocyte/macrophage population in DIO mice. Furthermore, 
      infusion of MCP-1 into lean mice increased the CD11b-positive monocyte population 
      without inducing changes in body weight. Given the importance of MCP-1 in 
      activation of monocytes and subsequent atherosclerotic development, these results 
      suggest a novel role of adiposity in the development of vascular disorders.
FAU - Takahashi, Kazuhiko
AU  - Takahashi K
AD  - Banyu Tsukuba Research Institute in collaboration with Merck Research 
      Laboratories, Tsukuba, Ibaraki 300-2611, Japan.
FAU - Mizuarai, Shinji
AU  - Mizuarai S
FAU - Araki, Hiromitsu
AU  - Araki H
FAU - Mashiko, Satoshi
AU  - Mashiko S
FAU - Ishihara, Akane
AU  - Ishihara A
FAU - Kanatani, Akio
AU  - Kanatani A
FAU - Itadani, Hiraku
AU  - Itadani H
FAU - Kotani, Hidehito
AU  - Kotani H
LA  - eng
PT  - Journal Article
DEP - 20030916
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CD11b Antigen)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adipose Tissue/chemistry/metabolism
MH  - Animals
MH  - Arteriosclerosis/etiology
MH  - CD11b Antigen/*analysis
MH  - Chemokine CCL2/blood/genetics/*physiology
MH  - Gene Expression Profiling
MH  - Macrophage Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/*cytology/immunology
MH  - Obesity/*blood/complications
MH  - RNA, Messenger/analysis
EDAT- 2003/09/18 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/09/18 05:00
PHST- 2003/09/18 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/09/18 05:00 [entrez]
AID - S0021-9258(20)75947-8 [pii]
AID - 10.1074/jbc.M309895200 [doi]
PST - ppublish
SO  - J Biol Chem. 2003 Nov 21;278(47):46654-60. doi: 10.1074/jbc.M309895200. Epub 2003 
      Sep 16.