PMID- 1314738
OWN - NLM
STAT- MEDLINE
DCOM- 19920526
LR  - 20190620
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 301
IP  - 2
DP  - 1992 Apr 20
TI  - Stoichiometric association of extrinsic cytochrome c550 and 12 kDa protein with a 
      highly purified oxygen-evolving photosystem II core complex from Synechococcus 
      vulcanus.
PG  - 145-9
AB  - A highly purified, native photosystem II (PS II) core complex was isolated from 
      thylakoids of Synechococcus vulcanus, a thermophilic cyanobacterium by 
      lauryldimethylamine N-oxide (LDAO) and dodecyl beta-D-maltoside solubilization. 
      This native PS II core complex contained, in addition to the proteins that have 
      been well characterized in the core complex previously purified by LDAO and 
      Triton X-100, two more extrinsic proteins with apparent molecular weights of 17 
      and 12 kDa. These two proteins were associated with the core complex in 
      stoichiometric amounts and could be released by treatment with 1 M CaCl2 or 1 M 
      alkaline Tris but not by 2 M NaCl or low-glycerol treatment, indicating that they 
      are the real components of PS II of this cyanobacterium. N-Terminal sequencing 
      revealed that the 17 and 12 kDa proteins correspond to the apoprotein of 
      cytochrome c550, a low potential c-type cytochrome, and the 9 kDa extrinsic 
      protein previously found in a partially purified PS II preparation from 
      Phormidium laminosum, respectively. In spite of retention of these two extrinsic 
      proteins, no homologues of higher plant 23 and 17 kDa extrinsic proteins could be 
      detected in this cyanobacterial PS II core complex.
FAU - Shen, J R
AU  - Shen JR
AD  - Solar Energy Research Group, Institute of Physical and Chemical Research (RIKEN), 
      Saitama, Japan.
FAU - Ikeuchi, M
AU  - Ikeuchi M
FAU - Inoue, Y
AU  - Inoue Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cytochrome c Group)
RN  - 0 (Photosynthetic Reaction Center Complex Proteins)
RN  - 9064-80-6 (cytochrome C-550)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/*chemistry
MH  - Cyanobacteria/*enzymology
MH  - Cytochrome c Group/*chemistry
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Molecular Sequence Data
MH  - Photosynthetic Reaction Center Complex Proteins/*chemistry
MH  - Sequence Alignment
MH  - Spectrum Analysis
EDAT- 1992/04/20 00:00
MHDA- 1992/04/20 00:01
CRDT- 1992/04/20 00:00
PHST- 1992/04/20 00:00 [pubmed]
PHST- 1992/04/20 00:01 [medline]
PHST- 1992/04/20 00:00 [entrez]
AID - 0014-5793(92)81235-E [pii]
AID - 10.1016/0014-5793(92)81235-e [doi]
PST - ppublish
SO  - FEBS Lett. 1992 Apr 20;301(2):145-9. doi: 10.1016/0014-5793(92)81235-e.