PMID- 1314872
OWN - NLM
STAT- MEDLINE
DCOM- 19920528
LR  - 20190509
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 165
IP  - 5
DP  - 1992 May
TI  - In vitro suppression of bone marrow progenitor cell differentiation by human 
      herpesvirus 6 infection.
PG  - 925-9
AB  - Suppression of marrow function may be one of the most serious effects of human 
      herpesvirus 6 (HHV-6) infection in marrow transplant patients. In this study, 
      normal bone marrow mononuclear cells were infected in vitro with HHV-6, and a 
      methylcellulose-based colony formation assay was used to evaluate the impact of 
      the infection on marrow cell differentiation and proliferation. Results 
      demonstrated that the outgrowth of colony-forming units of granulocyte and 
      macrophage lineages (cfu-GM) was decreased by approximately 43%, that growth of 
      cfu of granulocyte, erythrocyte, macrophage, and megakaryocyte lineages 
      (cfu-GEMM) was inhibited by an average of 71%, and that the erythroid 
      burst-forming unit (bfu-E) was decreased by approximately 73%. Further, outgrowth 
      of the marrow stromal layer was reduced 74%. Direct infection of bone marrow 
      monocytes was observed, although cell-free virus could not be detected in 
      infected culture supernatants. Addition of a neutralizing monoclonal antibody 
      specific for interferon-alpha to the infected cultures resulted in an almost 
      complete reversal of the viral suppressive effects.
FAU - Knox, K K
AU  - Knox KK
AD  - Department of Pathology, Medical College of Wisconsin, Milwaukee 53226.
FAU - Carrigan, D R
AU  - Carrigan DR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
SB  - IM
MH  - Bone Marrow/*microbiology/pathology
MH  - Cell Differentiation
MH  - Cell Division
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay
MH  - Fluorescent Antibody Technique
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Stem Cells/*microbiology/pathology
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
AID - 10.1093/infdis/165.5.925 [doi]
PST - ppublish
SO  - J Infect Dis. 1992 May;165(5):925-9. doi: 10.1093/infdis/165.5.925.