PMID- 1316543
OWN - NLM
STAT- MEDLINE
DCOM- 19920618
LR  - 20071114
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 6
IP  - 3
DP  - 1992 Mar
TI  - Two different genes coding for processable and nonprocessable forms of a viral 
      envelope protein can account for the apparent hormonal stimulation of protein 
      processing in W7MG1 lymphoma cells.
PG  - 459-67
AB  - In the mouse mammary tumor virus (MMTV)-infected mouse T-lymphoma cell line 
      W7MG1, glucocorticoid hormone regulates two aspects of MMTV gene expression: 
      hormone stimulates MMTV gene transcription and increases the ratio of mature 
      envelope proteins to envelope precursor protein produced. To separate these two 
      effects and determine the mechanism by which hormone regulates the conversion of 
      the envelope precursor Pr74 to the mature cleaved products gp52 and gp33, we 
      constructed expression vectors in which the envelope gene is constitutively 
      transcribed. Surprisingly, the envelope precursor protein Pr74 encoded by two 
      independently isolated, allelic envelope genes behaved differently. Pr74-P 
      (encoded by the ENV/P gene) was processed efficiently to the mature products gp52 
      and gp33, independently of the level of expression, hormonal induction of 
      cellular genes, or the presence of other MMTV proteins. In contrast, under the 
      same conditions, Pr74-N (encoded by the ENV/N gene) was not processed further 
      despite being relatively stable. In sucrose gradient analyses, Pr74-P sedimented 
      as monomers, whereas Pr74-N was found in high mol wt aggregates of heterogeneous 
      size. Coimmunoprecipitation analysis determined that Pr74-N associated with BiP, 
      whereas Pr74-P did not. This is indicative of improper folding of Pr74-N in the 
      endoplasmic reticulum.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Bedgood, R M
AU  - Bedgood RM
AD  - Department of Pathology, University of Southern California Health Sciences 
      Center, Los Angeles 90033.
FAU - Bahner, I
AU  - Bahner I
FAU - Kohn, D B
AU  - Kohn DB
FAU - Stallcup, M R
AU  - Stallcup MR
LA  - eng
GR  - AI-29125/AI/NIAID NIH HHS/United States
GR  - NS-26991/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (Glucocorticoids)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
GS  - ENV/N
GS  - ENV/P
MH  - Animals
MH  - Cloning, Molecular
MH  - Endoplasmic Reticulum/*metabolism
MH  - Genes, env/*physiology
MH  - Glucocorticoids/*physiology
MH  - Lymphoma, T-Cell
MH  - Mammary Tumor Virus, Mouse/genetics/*metabolism
MH  - Mice
MH  - Protein Conformation
MH  - Protein Processing, Post-Translational/genetics/physiology
MH  - Rats
MH  - Tumor Cells, Cultured
MH  - Viral Envelope Proteins/genetics/*metabolism
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
AID - 10.1210/mend.6.3.1316543 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1992 Mar;6(3):459-67. doi: 10.1210/mend.6.3.1316543.