PMID- 1317246
OWN - NLM
STAT- MEDLINE
DCOM- 19920701
LR  - 20181113
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 56
IP  - 2
DP  - 1992 Apr
TI  - A short incubation serum neutralization test for bovine viral diarrhea virus.
PG  - 161-4
AB  - A three day serum neutralization (SN) test for the detection of antibodies to 
      bovine viral diarrhea virus (BVDV), which is an improvement on the existing five 
      day test, is described. The improved test results in a more rapid viral 
      cytopathic effect and utilizes Madin Darby kidney (MDBK) cells, and horse serum 
      as a medium supplement. A comparison of tests utilizing the NADL and the Singer 
      strains of BVDV and the use of either secondary bovine kidney cells with calf 
      serum (BKCS) or continuous MDBK cells with horse serum (MDHS) was performed. 
      Analysis of the SN results of 685 serum samples from 445 Quebec and Ontario 
      cattle showed that there was no difference, as expected, in the means of the SN 
      antibody titers when the NADL strain was used in either the BKCS or MDHS system 
      but SN antibody titers were elevated (p less than 0.01) when the Singer strain 
      was used in the MDHS system. The SN test with the Singer strain also yielded 
      significantly higher titers for sera from 200 Alberta cattle.
FAU - Deregt, D
AU  - Deregt D
AD  - Animal Diseases Research Institute, Lethbridge, Alberta.
FAU - Smithson, S
AU  - Smithson S
FAU - Kozub, G C
AU  - Kozub GC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (Antibodies, Viral)
RN  - 0 (Culture Media)
SB  - IM
MH  - Alberta
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Bovine Virus Diarrhea-Mucosal Disease/*immunology
MH  - Cattle
MH  - Cell Line
MH  - Culture Media
MH  - Diarrhea Viruses, Bovine Viral/*immunology
MH  - *Neutralization Tests
MH  - Ontario
MH  - Quebec
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
PMC - PMC1263525
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
PMCR- 1992/07/01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
PHST- 1992/07/01 00:00 [pmc-release]
PST - ppublish
SO  - Can J Vet Res. 1992 Apr;56(2):161-4.