PMID- 131727
OWN - NLM
STAT- MEDLINE
DCOM- 19760802
LR  - 20190816
IS  - 0012-186X (Print)
IS  - 0012-186X (Linking)
VI  - 12
IP  - 2
DP  - 1976 May
TI  - Human adipose tissue in culture V. Studies on the metabolic effects of insulin.
PG  - 137-43
AB  - Specimens of human adipose tissue were cultured for one week with or without the 
      addition of insulin. The basal as well as the noradenaline-stimulated lipolysis 
      were enhanced in the explants cultured with insulin, showing that the long-term 
      effect of the hormone is lipolytic. However, an acute antilipolytic effect of 
      insulin could be demonstrated in these explants in the subsequent short-term 
      incubations. The basal rate of glucose incorporation into the lipids was enhanced 
      in the explants cultured with insulin. When insulin was added in the short-term 
      incubations these explants did not further respond to the hormone while this was 
      the case with the explants cultured without insulin. Thus, it seems that 
      prolonged exposure to insulin leads to a diminished acute effect of the hormone 
      on glucose metabolism. However, the same explants responded to the antilipolytic 
      effect showing that insulin was able to bind itself to the membrane. The 
      activities of hexokinase (HK), glucose-6-phosphage dehydrogenase (G6PDH), 
      pyruvate kinase (PK) and lactate dehydrogenase (LDH) were increased in large fat 
      cells both in freshly excised tissue and in cultured explants. However, the 
      activity of phosphofructokinase (PFK) did not correlate with the cell size. The 
      presence of insulin during the culture period enhanced the activities of G7PDH, 
      PK, and LDH, while this was not found for HK or PFK. The data thus suggest that 
      the metabolic capacity of human fat cells is enhanced by long-term exposure to 
      insulin. Although enzyme induction could be shown for G6PDH, PK and LDH it seems 
      unlikely that this is of importance for the increased rates of glucose metabolism 
      in these explants since the rate-limiting enzymes, HK and PGK, were not 
      increased. Most probably, then, this stimulating effect of insulin is exerted on 
      the membrane and the rate of glucose transport.
FAU - Smith, U
AU  - Smith U
FAU - Bostrom, S
AU  - Bostrom S
FAU - Johansson, R
AU  - Johansson R
FAU - Nyberg, G
AU  - Nyberg G
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Diabetologia
JT  - Diabetologia
JID - 0006777
RN  - 0 (Insulin)
RN  - 0 (Triglycerides)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - IY9XDZ35W2 (Glucose)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Adipose Tissue/*drug effects/metabolism
MH  - Adult
MH  - Aged
MH  - Culture Techniques
MH  - Glucose/*metabolism
MH  - Glucosephosphate Dehydrogenase/metabolism
MH  - Hexokinase/metabolism
MH  - Humans
MH  - Insulin/*pharmacology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - *Lipid Metabolism
MH  - Middle Aged
MH  - Norepinephrine/pharmacology
MH  - Phosphofructokinase-1/metabolism
MH  - Pyruvate Kinase/metabolism
MH  - Triglycerides/biosynthesis
EDAT- 1976/05/01 00:00
MHDA- 1976/05/01 00:01
CRDT- 1976/05/01 00:00
PHST- 1976/05/01 00:00 [pubmed]
PHST- 1976/05/01 00:01 [medline]
PHST- 1976/05/01 00:00 [entrez]
AID - 10.1007/BF00428979 [doi]
PST - ppublish
SO  - Diabetologia. 1976 May;12(2):137-43. doi: 10.1007/BF00428979.